BD Horizon™ Real Fluorochromes

Minimize Cross-laser Spillover. Optimize Panel Resolution.

Format	Spectral	Conventional	Spillover (1-low, 4-high)	Alternative To
Violet Laser
RV828	✓	‒	1	Qdot^™ 800, NovaFluor^™ V800
Blue Laser
RB545	✓	‒	1	(Unique position)
RB613	✓	✓	2	PE-CF594, PE-Dazzle^™ 594, BB630-P2, StarBright^™ Blue 615
RB670	✓	✓	2	PE-Cy5, BB660-P2, StarBright^™ Blue 675
RB705	✓	✓	2	PerCP-Cy5.5, BB700, StarBright^™ Blue 700
RB744	✓	✓	1	BB755-P
RB780	✓	✓	1	PE-Cy7, BB790-P
RB824	✓	✓	1	PerCP/Fire^™806, StarBright^™Blue 810
Yellow-Green Laser
RY586	✓	✓	1	PE
RY610	✓	✓	1	PE-CF594, PE-Dazzle^™ 594
RY655	✓	✓	3	PE-Cy5, StarBright Yellow 665
RY703	✓	✓	2	PE-Cy5.5, StarBright^™ Yellow 720
RY743	✓	✓	2	PE/Fire^™ 744
RY775	✓	✓	2	PE-Cy7, StarBright^™ Yellow 800
Red Laser
RR688	✓	‒	1	Alexa Fluor^™ 660, Spark NIR^™685, NovaFluor^™ R685, cFluor^™ R685

“The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

Dr. Florian Ebner

Distinguished Lab Scientist

Cancer Pharmacology and Disease Positioning

“I’m really excited about the BD Horizon RealBlue^™ 780 Dye. The staining we have done so far with Ki-67 and Granzyme B can be seen very clearly, even with high dilutions.”

Johannes Brandi

Researcher

Bernhard Nocht Institute for Tropical Medicine

"BD Horizon RealYellow™ 586 (RY586) Reagents look like the best alternative for PE and will simplify panel design on conventional flow cytometers. They can also be an additional dye for spectral panel design.”

Gert Van Isterdael, PhD

VIB Ghent University, Belgium