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      Cartridge Reagent Kit

      BD Rhapsody™ Cartridge Reagent Kit

      (RUO)
      제품 세부 정보
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      BD Rhapsody™
      Single cell multiomics experiments on the BD Rhapsody™ platform
      RUO
      Rhapsody Cartridge Reagent Kit


      설명

      The purpose of this kit is to provide the necessary reagents needed to prepare the BD Rhapsody™ Cartridges, extract RNA and antibody oligos bound to the cells, and capture them on BD Rhapsody™ Cell Capture Beads.Learn more about the intended use of this kit and the associated protocol from the BD Rhapsody™ Single-Cell Analysis System user guide .

      633731 Rev. 1
      인용 및 참고 문헌
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      개발 참고 자료 (28)

      1. Alivernini S, MacDonald L, Elmesmari A, et al. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis. Nat Med. 2020; 26(8):1295-1306. (Biology). 참조 보기
      2. Arroyo Hornero R, Georgiadis C, Hua P, et al. CD70 expression determines the therapeutic efficacy of expanded human regulatory T cells. Commun Biol. 2020; 3(1):375. (Biology). 참조 보기
      3. De Domenico E, Bonaguro L, Schulte-Schrepping J, Becker M, Händler K, Schultze JL. Optimized workflow for single-cell transcriptomics on infectious diseases including COVID-19. STAR Protoc. 2020; 1(3):100233. (Biology). 참조 보기
      4. Erickson JR, Mair F, Bugos G, et al. AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level. STAR Protoc. 2020; 1(2):100092. (Biology). 참조 보기
      5. Ferreira RC, Castro Dopico X, Oliveira JJ, et al. Chronic Immune Activation in Systemic Lupus Erythematosus and the Autoimmune PTPN22 Trp620 Risk Allele Drive the Expansion of FOXP3+ Regulatory T Cells and PD-1 Expression. Front Immunol. 2019; 10:2606. (Biology). 참조 보기
      6. Gertz ML, Chin CR, Tomoiaga D, et al. Multi-omic, Single-Cell, and Biochemical Profiles of Astronauts Guide Pharmacological Strategies for Returning to Gravity. Cell Rep. 2020; 33(10):108429. (Biology). 참조 보기
      7. Hasegawa T, Kikuta J, Sudo T, et al. Identification of a novel arthritis-associated osteoclast precursor macrophage regulated by FoxM1. Nat Immunol. 2019; 20(12):1631-1643. (Biology). 참조 보기
      8. Jensen-Cody SO, Flippo KH, Claflin KE, et al. FGF21 Signals to Glutamatergic Neurons in the Ventromedial Hypothalamus to Suppress Carbohydrate Intake. Cell Metab. 2020; 32(2):273-286. (Biology). 참조 보기
      9. Li H, Wang H, Sokulsky L, et al. Single-cell transcriptomic analysis reveals key immune cell phenotypes in the lungs of patients with asthma exacerbation. J Allergy Clin Immunol. 2020; S0091(20):31400-7. (Biology). 참조 보기
      10. Li S, Simoni Y, Becht E, et al. Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal Cancer. Cell Rep Med. 2020; 1(3):100039. (Biology). 참조 보기
      11. Li Z, Li M, Shi SX, et al. Brain transforms natural killer cells that exacerbate brain edema after intracerebral hemorrhage. J Exp Med. 2020; 217(12):e20200213. (Biology). 참조 보기
      12. Mair F, Erickson JR, Voillet V, et al. A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level. Cell Rep. 2020; 31(1):107499. (Biology). 참조 보기
      13. Malkani S, Chin CR, Cekanaviciute E, et al. Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development. Cell Rep. 2020; 33(10):108448. (Biology). 참조 보기
      14. Mohebiany AN, Ramphal NS, Karram K, et al. Microglial A20 Protects the Brain from CD8 T-Cell-Mediated Immunopathology. Cell Rep. 2020; 30(5):1585-1597. (Biology). 참조 보기
      15. Sainson RCA, Thotakura AK, Kosmac M, et al. An Antibody Targeting ICOS Increases Intratumoral Cytotoxic to Regulatory T-cell Ratio and Induces Tumor Regression. Cancer Immunol Res. 2020; 8(12):1568-1582. (Biology). 참조 보기
      16. Schulte-Schrepping J, Reusch N, Paclik D, et al. Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment. Cell. 2020; 182(6):1419-1440. (Biology). 참조 보기
      17. Song B, Xiong G, Luo H, Zuo Z, Zhou Z, Chang X. Single-cell RNA sequencing of mouse neural stem cell differentiation reveals adverse effects of cadmium on neurogenesis. Food Chem Toxicol. 2021; (148):111936. (Biology). 참조 보기
      18. Song L, Zhang S, Yu S, et al. Cellular heterogeneity landscape in laryngeal squamous cell carcinoma. Int J Cancer. Int J Cancer. 2020; 147(10):2879-2890. (Biology). 참조 보기
      19. Sun H, Wen X, Li H, et al. Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration. Ann Rheum Dis. 2020; 79(3):408-417. (Biology). 참조 보기
      20. Tang X, Zhang S, Peng Q, et al. Sustained IFN-I stimulation impairs MAIT cell responses to bacteria by inducing IL-10 during chronic HIV-1 infection. Sci Adv. 2020; 6(8):eaaz0374. (Biology). 참조 보기
      21. Trzupek D, Dunstan M, Cutler AJ, et al. Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis. Genome Med. 2020; 12(1):55. (Biology). 참조 보기
      22. Wagar LE, Salahudeen A, Constantz CM, et al. Modeling human adaptive immune responses with tonsil organoids. Nat Med.. 2021; 27(1):125-135. (Biology). 참조 보기
      23. Wen M, Cai G, Ye J, Liu X, Ding H, Zeng H. Single-cell transcriptomics reveals the alteration of peripheral blood mononuclear cells driven by sepsis. Ann Transl Med. 2020; 8(4):125. (Biology). 참조 보기
      24. Wu B, Wang W, Wang H, et al. Single-Cell Sequencing of Glioblastoma Reveals Central Nervous System Susceptibility to SARS-CoV-2. Front Oncol. 2020; (10):566599. (Biology). 참조 보기
      25. Wu M, Wang C, Shi B, et al. A novel recombinant cccDNA-based mouse model with long term maintenance of rcccDNA and antigenemia. Antiviral Res. 2020; 180:104826. (Biology). 참조 보기
      26. Xie X, Shi Q, Wu P, et al. Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection.. Nat Immunol. 2020; 21(9):1119-1133. (Biology). 참조 보기
      27. Yang Y, Liu F, Liu W, et al. Analysis of single-cell RNAseq identifies transitional states of T cells associated with hepatocellular carcinoma. Clin Transl Med. 2020; 10(3):e133. (Biology). 참조 보기
      28. Zhao L, Yao C, Xing X, et al. Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells. Nat Commun. 2020; 11(1):5683. (Biology). 참조 보기
      모두 보기 (28) 간단히 보기
      633731 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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