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Western blot analysis of p53 on A431 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of p53.
A431
BD Transduction Laboratories™ Purified Mouse Anti-p53
BD Transduction Laboratories™ Purified Mouse Anti-p53
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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The p53 protein is critical to regulation of normal cell growth and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. Mutations and/or allelic loss of the p53 gene is associated with a wide variety of human tumors. Known to have a role in transcriptional regulation, p53 suppresses various promoters containing TATA elements in an apparently sequence-independent fashion. p53 also binds to DNA in a sequence-specific manner via recognition of a 20 bp consensus-binding site. This interaction stimulates the expression of genes downstream of the p53 binding site. A number of genes that contain p53-binding sites have been identified, including MDM2, GADD45, and muscle creatine kinase. It is thought that MDM2 is a feedback regulator of p53. In addition, a p53-inducible gene, Cip1, has been identified and shown to suppress tumor cell growth in culture.
개발 참고 자료 (5)
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Hurd C, Khattree N, Alban P. Hormonal regulation of the p53 tumor suppressor protein in T47D human breast carcinoma cell line. J Biol Chem. 1995; 270(48):28507-28510. (Clone-specific: Western blot). 참조 보기
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Mercer WE. Cell cycle regulation and the p53 tumor suppressor protein. Crit Rev Eukaryot Gene Expr.. 1992; 2(3):251-263. (Biology). 참조 보기
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Natsugoe S, Nakashima S, Matsumoto M. Expression of p21WAF1/Cip1 in the p53-dependent pathway is related to prognosis in patients with advanced esophageal carcinoma. Clin Cancer Res. 1999; 5(9):2445-2449. (Clone-specific: Immunohistochemistry). 참조 보기
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Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Clone-specific: Immunofluorescence). 참조 보기
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Stahnke K, Fulda S, Friesen C, Strauss G, Debatin KM. Activation of apoptosis pathways in peripheral blood lymphocytes by in vivo chemotherapy. Blood. 2001; 98(10):3066-3073. (Clone-specific: Flow cytometry, Western blot). 참조 보기
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