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Western blot analysis of CD11a (Integrin αL) on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti-CD11a antibody.
BD Pharmingen™ Purified Mouse Anti-Human CD11a
규제 상태 범례
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준비 및 보관
권장 분석 절차
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
CD11a, also known as integrin αL or LFA-1 (lymphocyte function-associated antigen-1), is a member of the β2 family of integrins that includes Mac-1 and p150,95. It is composed of two noncovalently linked α and β chains which are associated with the actin cytoskeleton. CD11a (integrin αL) has been reported to be expressed on the surface of T cells, neutrophils, and macrophages. It is involved in cell-cell adhesion and it also conveys signals from the plasma membrane to the cytoplasm. Activation of the TCR/CD3 complex on T cells transmits intracellular signals that activate integrin αL ("inside-out" signaling). This results in the conformational change of integrin αL, which increases its affinity for its ligand ICAM-1,-2, and -3. Furthermore, this signaling releases integrin αL from the actin cytoskeleton, resulting in clustering of this surface molecule. The high affinity conformation and the clustering of integrin αL increases the adhesion between it and ICAM. This adhesion is essential for leukocyte activation and results in "outside-in" signaling which induces the polymerization of actin, thereby stabilizing the integrin αL-ICAM interaction. This interaction is necessary for CTL and NK mediated killing, T and B cell responses, ADCC, and immune cell extravasation.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
개발 참고 자료 (4)
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Larson RS, Corbi AL, Berman L, Springer T. Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily. J Cell Biol. 1989; 108(2):703-712. (Biology). 참조 보기
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Lub M, van Kooyk Y, van Vliet SJ, Figdor CG. Dual role of the actin cytoskeleton in regulating cell adhesion mediated by the integrin lymphocyte function-associated molecule-1. Mol Biol Cell. 1997; 8(2):341-351. (Biology). 참조 보기
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Mamane Y, Grandvaux N, Hernandez E, et al. Repression of IRF-4 target genes in human T cell leukemia virus-1 infection. Nature. 2002; 21(44):6751-6765. (Biology: Western blot). 참조 보기
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Nueda A, Lopez-Cabrera M, Vara A, Corbi AL. Characterization of the CD11a (alpha L, LFA-1 alpha) integrin gene promoter. J Biol Chem. 1993; 268(26):19305-19311. (Biology). 참조 보기
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