Purified Mouse Anti-GFAP

BD Transduction Laboratories™ Purified Mouse Anti-GFAP

Name: Purified Mouse Anti-GFAP
Brand: BD Transduction Laboratories™
SKU: 610565

Clone 52/GFAP

(RUO)

Western blot analysis of GFAP on SW13 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-GFAP antibody.

Immunofluroescent staining of Rabbit Brain tissue.

Western blot analysis of GFAP on SW13 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-GFAP antibody.

Immunofluroescent staining of Rabbit Brain tissue.

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Scientific Resources

제품 세부 정보

Brand: BD Transduction Laboratories™

Product Name: GFAP Pure 52/GFAP 50ug

Reactivity: Human (QC Testing), Mouse, Rat, Chicken (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Human GFAP aa. 418-432

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)

Target Molecular Weight: 50 kDa

Concentration: 250 µg/ml

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

610565 Rev. 1

항체 세부 정보

52/GFAP

The cytoskeleton is an array of highly ordered filaments that maintains the shape, structure, and functionality of cells. There are three major types of filaments: actin filaments, microtubules, and intermediate filaments. The distinctions among them are based on protein composition and arrangements. Glial fibrillary acidic protein (GFAP) is a 50 kDa component of the intermediate filaments and was originally identified in astrocytes. The overall structure of GFAP, which confirms its similarities to other intermediate filament proteins, indicates a central rod of about 130 amino acids flanked by coiled regions. GFAP has been extensively used as an immunohistochemical marker for tumors derived from astroglia.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610565 Rev. 1

형광 세부 정보

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

형광: Purified

610565 Rev.1

인용 및 참고 문헌

개발 참고 자료 (1)

Reeves SA, Helman LJ, Allison A, Israel MA. Molecular cloning and primary structure of human glial fibrillary acidic protein. Proc Natl Acad Sci U S A. 1989; 86(13):5178-5182. (Biology). 참조 보기

610565 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.