Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Western blot analysis of 14-3-3 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1: 1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Mouse Anti- 14-3-3 antibody.
Immunofluorescence staining of SKN cells (Human neuroblastoma).
BD Transduction Laboratories™ Purified Mouse Anti- 14-3-3
BD Transduction Laboratories™ Purified Mouse Anti- 14-3-3
규제 상태 범례
Becton, Dickinson and Company의 명시적인 서면 승인 없이는 사용 하실 수 없습니다.
준비 및 보관
권장 분석 절차
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The family of 14-3-3 proteins has been implicated in numerous cellular processes such as neurotransmission, phospholipase activity, and calcium signaling. The 14-3-3 protein can associate with several proteins involved in cell proliferation, such as Raf-1, Bcr-Abl, middle T antigen, and CDC25 phosphatase. It appears that 14-3-3 proteins enhance the kinase activity of the Raf proto-oncogene equally as well as Ras, thus, implicating 14-3-3 proteins in the Raf/Ras/Map kinase signaling pathway. However, in spite of 14-3-3 direct association with the CDC25 phosphatase, this interaction does not increase the phosphatase activity. It has been reported that this antibody detects 14-3-3 subunits β, γ, η, θ, and ζ , but not ε nor σ.
개발 참고 자료 (5)
-
Ayllon V, Cayla X, Garcia A, et al . Bcl-2 targets protein phosphatase 1 alpha to Bad. J Immunol. 2001; 166(12):7345-7352. (Biology: Immunoprecipitation, Western blot). 참조 보기
-
Conklin DS, Galaktionov K, Beach D.. 14-3-3 proteins associate with cdc25 phosphatases.. Proc Natl Acad Sci U S A. 1995; 92(17):7892-7896. (Biology). 참조 보기
-
Freed E, Symons M, Macdonald SG, McCormick F, Ruggieri R.. Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation.. Science. 1994 ; 265(5179):1713-1716. (Biology: Western blot). 참조 보기
-
Mamane Y, Grandvaux N, Hernandez E, et al. Repression of IRF-4 target genes in human T cell leukemia virus-1 infection. Nature. 2002; 21(44):6751-6765. (Biology: Immunofluorescence, Immunohistochemistry, Western blot). 참조 보기
-
Ostrerova N, Petrucelli L, Farrer M, Mehta N,. alpha-Synuclein shares physical and functional homology with 14-3-3 proteins.. J Neurosci. 1999; 19(14):5782-5791. (Biology: Immunoprecipitation, Western blot). 참조 보기
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.