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Immunofluorescence staining of iNOS/NOS Type II on mouse macrophages (RAW 264.7) treated with 10 ng/mL IFNγ + 1 µg/mL LPS for 12 hrs.
BD Transduction Laboratories™ FITC Mouse Anti-iNOS/NOS Type II
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-γ, TNF-α, and IL-1) and microbial products (lipopolysaccharide (LPS)), iNOS is activated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFκB. Data indicates that TGF-β affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain.
개발 참고 자료 (5)
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Angulo I, de las Heras FG, Garcia-Bustos JF, Gargallo D, Munoz-Fernandez MA, Fresno M. Nitric oxide-producing CD11b(+)Ly-6G(Gr-1)(+)CD31(ER-MP12)(+) cells in the spleen of cyclophosphamide-treated mice: implications for T-cell responses in immunosuppressed mice. Blood. 2000; 95(1):212-220. (Biology: Flow cytometry). 참조 보기
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Koprowski H, Zheng YM, Heber-Katz E, et al. In vivo expression of inducible nitric oxide synthase in experimentally induced neurologic diseases. Proc Natl Acad Sci U S A. 1993; 90(7):3024-3027. (Biology). 참조 보기
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Moulian N, Truffault F, Gaudry-Talarmain YM, Serraf A, Berrih-Aknin S. In vivo and in vitro apoptosis of human thymocytes are associated with nitrotyrosine formation. Blood. 2001; 97(11):3521-3530. (Biology: Immunofluorescence). 참조 보기
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Siedlar M, Mytar B, Krzeszowiak A, et al. Demonstration of iNOS-mRNA and iNOS in human monocytes stimulated with cancer cells in vitro. J Leukoc Biol. 1999; 65(5):597-604. (Biology: Flow cytometry, Immunofluorescence). 참조 보기
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Xie QW, Cho HJ, Calaycay J, et al. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science. 1992; 256(5054):225-228. (Biology). 참조 보기
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