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권장 분석 절차
ELISA: Purified Rat Anti-Mouse IL-10 antibody (Clone JES5-2A5, Cat. No. 551215) has been reported to be useful as a capture antibody in sandwich ELISA for mouse IL-10. Purified JES5-2A5 antibody can be paired with the Biotin Rat Anti-Mouse IL-10 antibody (Clone SXC-1, Cat. No. 554423) or Biotin Rat Anti-Mouse IL-10 antibody (Clone JES5-16E3, Cat. No. 554465) and recombinant mouse IL-10 (Cat. No. 550070) as the standard. Purified JES5-2A5 antibody should be titrated (suggested 2-6 µg/mL) to determine the optimal concentration for ELISA capture. An appropriate range of concentrations of the mouse IL-10 standard for obtaining a linear standard curve is 2 ng/mL to 15 pg/mL mouse IL-10.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The JES5-2A5 antibody reacts with mouse IL-10. The immunogen used to generate the JES5-2A5 hybridoma was E. coli-expressed mouse IL-10. This is a neutralizing antibody.
개발 참고 자료 (5)
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Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). 참조 보기
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Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Neutralization). 참조 보기
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Mo XY, Sarawar SR, Doherty PC. Induction of cytokines in mice with parainfluenza pneumonia. J Virol. 1995; 69(2):1288-1291. (Clone-specific: ELISA). 참조 보기
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Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific: ELISA). 참조 보기
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Sarawar SR, Sangster M, Coffman RL, Doherty PC. Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype. J Immunol. 1994; 153(3):1246-1253. (Clone-specific: ELISA). 참조 보기
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