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CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with purified mAb 17A2 (bottom left panel) and PE-conjugated anti-mouse CD19 mAb 1D3 (top left & bottom left panels), followed by FITC-conjugated anti-rat IgG2b mAb G15-337 (Cat. No. 553884) (top left & bottom left panels). BALB/c thymocytes were also stained with 17A2 mAb (bottom right panel), followed by FITC-conjugated anti-rat IgG2b (top right & bottom right panels). Flow cytometry was performed on a BD FACSCan™ flow cytometry system.
BD Pharmingen™ Purified Rat Anti-Mouse CD3 Molecular Complex
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준비 및 보관
권장 분석 절차
Precautions: Polyclonal goat anti-rat Ig (Cat. No. 554016) has been reported to have weak reactivity to to this rat IgG2b mAb. Therefore, the use of FITC-conjugated anti-rat IgG2b mAb G15-337 (Cat. No. 553884) is recommended as a second step for flow cytometry.
Note: The hamster anti-mouse CD3e mAb 145-2C11 (Cat. No. 553057) has been reported to induce greater functional responses than 17A2 antibody in assays which depend on presentation of the monoclonal antibody by Fc receptor-positive macrophages.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
개발 참고 자료 (3)
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Miescher GC, Schreyer M, MacDonald HR. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989; 23(2):113-118. (Immunogen: Cytotoxicity, Functional assay, Immunohistochemistry, Immunoprecipitation, Stimulation). 참조 보기
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Mysliwietz J, Thierfelder S. Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient. Blood. 1992; 80(10):2661-2667. (Clone-specific: Depletion). 참조 보기
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Wu L, Antica M, Johnson GR, Scollay R, Shortman K. Developmental potential of the earliest precursor cells from the adult mouse thymus. J Exp Med. 1991; 174(6):1617-1627. (Biology: Cytotoxicity). 참조 보기
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