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Two-color analysis of CD172a expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified rat IgG1 isotype control mAb (left panel) or purified mAb P84 (right panel), followed by FITC-conjugated anti-rat IgG1 mAb RG11/39.4 (Cat. No. 553892, both panels), then PE-conjugated anti-mouse CD11b (Mac-1) mAb M1/70 (Cat. No. 557397/553311). Total viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCaliburª (BD Biosciences, San Jose, CA).
BD Pharmingen™ Purified Rat Anti-Mouse CD172a
규제 상태 범례
Becton, Dickinson and Company의 명시적인 서면 승인 없이는 사용 하실 수 없습니다.
준비 및 보관
권장 분석 절차
Other reported applications include immunofluorescent staining of cell monolayers and frozen tissue sections, immunoaffinity purification of CD172a, blocking of cellular attachment to CD172a, immunoelectron microscopy, immunoprecipitation, and enhancement of phagocytosis by bone marrow-derived macrophages. It is not effective for western blot analysis.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
The P84 monoclonal antibody specifically binds to CD172a, also known as SIgnal-Regulatory Protein α (SIRPα), Src Homology 2 domain-containing protein tyrosine Phosphatase (SHP) Substrate 1 (SHPS-1), or Brain Immunoglobulin-like molecule with Tyrosine-based activation motifs (BIT). CD172a is an adhesion molecule of the Ig superfamily which is expressed on neurons in the central nervous system and the retina, on macrophages, and on bone-marrow myeloid cells. Its ligand, CD47, or Integrin-Associated Protein (IAP), is expressed by a wide variety of cells. CD172a and CD47 are proposed to mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages.
개발 참고 자료 (7)
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Chuang W, Lagenaur CF. Central nervous system antigen P84 can serve as a substrate for neurite outgrowth. Dev Biol. 1990; 137(2):219-232. (Immunogen: Blocking, Immunofluorescence). 참조 보기
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Comu S, Weng W, Olinsky S. The murine P84 neural adhesion molecule is SHPS-1, a member of the phosphatase-binding protein family. J Neurosci. 1997; 17(22):8702-8710. (Clone-specific: Immunofluorescence). 참조 보기
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Gresham HD, Dale BM, Potter JW, et al. Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr. J Exp Med. 2000; 191(3):515-528. (Clone-specific: Immunoprecipitation). 참조 보기
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Jiang P, Lagenaur CF, Narayanan V. Integrin-associated protein is a ligand for the P84 neural adhesion molecule. J Biol Chem. 1999; 274(2):559-562. (Biology). 참조 보기
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Mi ZP, Jiang P, Weng WL, Lindberg FP, Narayanan V, Lagenaur CF. Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina. J Comp Neurol. 2000; 416(3):335-344. (Clone-specific: Electron microscopy, Immunofluorescence). 참조 보기
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Oldenborg PA, Zheleznyak A, Fang YF, Lagenaur CF, Gresham HD, Lindberg FP. Role of CD47 as a marker of self on red blood cells. Science. 2000; 288(5473):2051-2054. (Biology). 참조 보기
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Veillette A, Thibaudeau E, Latour S. High expression of inhibitory receptor SHPS-1 and its association with protein-tyrosine phosphatase SHP-1 in macrophages. J Biol Chem. 1998; 273(35):22719-22728. (Biology). 참조 보기
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