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The CX5 monoclonal antibody specifically binds to NKG2D, also known as CD314. NKG2D is a lectin-like receptor that is detected on resting and IL-2-activated NK cells, activated CD8-positive T lymphocytes, and LPS-activated macrophages, but not on resting T cells or unstimulated macrophages. NKG2D has little homology to the other members of the NKG2 family, NKG2A, C, and E, and does not form heterodimers with CD94. On NK cells, NKG2D is an activating receptor that associates with DAP10, an adapter protein that stimulates the PI3 kinase pathway. An isoform of mouse NKG2D can also associate with the signaling adapter protein DAP12, also known as KARAP (Killer cell-Associated Receptor-Associated Polypeptide), that activates the Syk and ZAP70 tyrosine kinases. On cytotoxic T cells, NKG2D is a co-stimulatory receptor that associates with DAP10. The ligands for NKG2D include the minor histocompatibility antigen H60, MULT1 (Murine UL16-binding protein-Like Transcript 1), and the five retinoic acid-inducible proteins Rae-1α, β, γ, δ and ε. Interactions of NKG2D with its ligands are involved in the regulation of innate and immune cytotoxic responses to tumor and pathogen-infected cells and in diabetes progression in the NOD mouse. The CX5 mAb blocks the binding of NKG2D to its ligands.
개발 참고 자료 (8)
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Carayannopoulos LN, Naidenko OV, Fremont DH, Yokoyama WM. Murine UL16–binding protein–like transcript 1: A newly described transcript encoding a high–affinity ligand for murine NKG2D . J Immunol. 2002; 169(8):4079-4083. (Biology). 참조 보기
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Cerwenka A, Bakker AB, McClanahan T, et al. Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice. Immunity. 2000; 12(6):721-727. (Biology). 참조 보기
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Diefenbach A, Jamieson AM, Liu SD, Shastri N, Raulet DH. Ligands for the murine NKG2D receptor: expression by tumor cells and activation of NK cells and macrophages. Nat Immunol. 2000; 1(2):119-126. (Biology). 참조 보기
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Diefenbach A, Tomasello E, Lucas M, et al. Selective associations with signaling proteins determine stimulatory versus costimulatory activity of NKG2D. Nat Immunol. 2002; 3(12):1142-1149. (Biology). 참조 보기
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Gilfillan S, Ho EL, Cella M, Yokoyama WM, Colonna M. NKG2D recruits two distinct adapters to trigger NK cell activation and costimulation . Nat Immunol. 2002; 3(12):1150-1155. (Biology). 참조 보기
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Ogasawara K, Hamerman JA, Ehrlich LR, et al. NKG2D blockade prevents autoimmune diabetes in NOD mice. Immunity. 2004; 20(6):757-767. (Clone-specific: Blocking). 참조 보기
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Ogasawara K, Hamerman JA, Hsin H, et al. Impairment of NK cell function by NKG2D modulation in NOD mice. Immunity. 2003; 18(1):41-51. (Immunogen: Blocking). 참조 보기
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Yokoyama WM. Natural killer cell receptors . Curr Opin Immunol. 1998; 10:298-305. (Biology).
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