Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Korea (Korea)
Profile Icon Profile Icon
로그인 / 등록 UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified NA/LE Rat Anti-Mouse CD106
      제품 세부 정보
      Down Arrow Up Arrow


      BD Pharmingen™
      Ms CD106 NALE 429 (MVCAM.A) 500ug
      Vcam-1; Vascular cell adhesion molecule 1; Vascular cell adhesion protein 1
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG2a, κ
      Mouse preadipose cell line PA6
      Flow cytometry (Routinely Tested), Blocking, Immunofluorescence, Immunohistochemistry-frozen, Immunoprecipitation (Reported)
      1.0 mg/ml
      22329
      AB_394785
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      준비 및 보관

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
      자세히 보기 blue down arrow 간략히 보기 blue up arrow

      제품 고시

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      자세히 보기 blue down arrow 간략히 보기 blue up arrow
      553329 Rev. 11
      항체 세부 정보
      Down Arrow Up Arrow
      429 (MVCAM.A)

      The 429 monoclonal antibody specifically binds to both the long (~110 kDa) transmembrane-spanning form and the truncated (~47 kDa) GPI-linked form of vascular cell adhesion molecule-1 (VCAM-1, CD106). CD106 is constitutively expressed on bone marrow stromal cells, myeloid cells, and splenic dendritic cells. Its expression on endothelial cells is upregulated by inflammatory cytokines and in certain pathologic conditions. CD106 expression has also been detected on apoptotic thymocytes, splenocytes, and lymphoid cell lines. VCAM-1 is a counter-receptor for VLA-4 (α4β1 integrin) and LPAM-1 (α4β7 integrin), and the 429 antibody partially blocks VCAM-1-mediated binding functions. Source of the immunogen was the mouse preadipose cell line PA6.

      553329 Rev. 11
      형광 세부 정보
      Down Arrow Up Arrow
      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      553329 Rev.11
      인용 및 참고 문헌
      Down Arrow Up Arrow

      개발 참고 자료 (6)

      1. Baron JL, Reich EP, Visintin I, Janeway CA Jr. The pathogenesis of adoptive murine autoimmune diabetes requires an interaction between alpha 4-integrins and vascular cell adhesion molecule-1. J Clin Invest. 1994; 93(4):1700-1708. (Clone-specific: Blocking, Immunohistochemistry). 참조 보기
      2. Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol. 1993; 11:767-804. (Biology). 참조 보기
      3. Buck CA, Edelman JM, Buck CE, Kennedy G, Baldwin HS. Expression patterns of adhesion receptors in the developing mouse lung: functional implications. Cell Adhes Commun. 1996; 4(2):69-87. (Clone-specific: Immunofluorescence). 참조 보기
      4. Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994; 20(1):25-44. (Clone-specific: Immunohistochemistry). 참조 보기
      5. Kinashi T, St Pierre Y, Springer TA. Expression of glycophosphatidylinositol-anchored and -non-anchored isoforms of vascular cell adhesion molecule 1 in murine stromal and endothelial cells. J Leukoc Biol. 1995; 57(1):168-173. (Immunogen: Immunoprecipitation). 참조 보기
      6. Koni PA, Joshi SK, Temann UA, Olson D, Burkly L, Flavell RA. Conditional vascular cell adhesion molecule 1 deletion in mice: impaired lymphocyte migration to bone marrow. J Exp Med. 2001; 193(6):741-754. (Clone-specific: Immunohistochemistry). 참조 보기
      모두 보기 (6) 간단히 보기
      553329 Rev. 11

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback