BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD93

Name: Purified NA/LE Mouse Anti-Human CD93
Brand: Purified NA/LE Mouse Anti-Human CD93
SKU: 552954

Clone R139 (RUO)

Expression of CD93 by unstimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stained with the purified NA/LE Mouse Anti-Human CD93 antibody (Cat. No. 552954, shaded histogram), or Purified NA/LE Mouse IgG2b, κ Isotype Control (Cat. No. 559530, open histogram). Secondary staining was carried out with Biotin Goat Anti-Mouse Ig secondary antibody (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061). Fluorescence histograms depicting CD93 (or Ig Isotype Control) expression were derived from gated events with the side and forward light-scatter characteristics of viable PBMCs.

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제품 세부 정보

Brand: BD Pharmingen™

Alternative Name: C1QR1; C1qRP; C1qR(P); C1q/MBL/SPA Receptor; MXRA4; GR11; Dj737e23.1

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG2b, κ

Immunogen: Clq-CLF-binding proteins

Application: Flow cytometry (Routinely Tested), Neutralization (Reported)

Concentration: 1.0 mg/ml

RRID: AB_394531

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

준비 및 보관

Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

관련 제품

Stain Buffer (FBS) RUO

사이즈 500 ML 카탈로그 No 554656

Stain Buffer (BSA) RUO

사이즈 500 ML 카탈로그 No 554657

Purified NA/LE Mouse IgG2b, κ Isotype Control RUO

사이즈 0.5 mg 카탈로그 No 559530

Biotin Goat Anti-Mouse Ig (Multiple Adsorption) RUO

사이즈 0.5 mg 카탈로그 No 553999

PE Streptavidin RUO

사이즈 0.5 mg 카탈로그 No 554061

552954 Rev. 3

항체 세부 정보

R139

The R139 monoclonal antibody specifically binds to CD93 which is also known as Complement component C1q receptor (C1qR), C1q receptor 1 (C1qR1), or Matrix-remodeling-associated protein 4 (MXRA4). The immunogen used to generate the R139 hybridoma was a preparation of CD93 protein. Human CD93 is a transmembrane glycoprotein that is highly expressed on monocytes, macrophages, granulocytes, and endothelial cells but not on T and B lymphocytes. CD93 is also known as the C1q/MBL/SPA Receptor as it binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). Human C1qRp is involved in the C1q-mediated enhancement of phagocytosis. R139 is suitable to detect CD93 expression on cells of myeloid lineage by flow cytometry, and CD93 in cellular lysates by Western blotting or immunoprecipitation. In addition, R139 reportedly neutralizes C1q-mediated enhancement of phagocytosis. CD93 has also been reported to define a human stem cell population with hematopoietic and hepatic potential.

552954 Rev. 3

형광 세부 정보

NA/LE

Purified NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin. Purified NA/LE antibodies are well suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

형광: NA/LE

552954 Rev.3

인용 및 참고 문헌

View product citations for antibody "552954" on CiteAb

개발 참고 자료 (8)

Danet GH, Luongo JL, Butler G, et al. C1qRp defines a new human stem cell population with hematopoietic and hepatic potential.. Proc Natl Acad Sci USA. 2002; 99(16):10441-5. (Biology). 참조 보기
Guan E, Robinson SL, Goodman EB, Tenner AJ. Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis. J Immunol. 1994; 152(8):4005-4016. (Immunogen). 참조 보기
Guan EN, Burgess WH, Robinson SL, Goodman EB, McTigue KJ, Tenner AJ. Phagocytic cell molecules that bind the collagen-like region of C1q. Involvement in the C1q-mediated enhancement of phagocytosis. J Biol Chem. 1991; 266(30):20345-20355. (Immunogen). 참조 보기
Nepomuceno RR, Henschen-Edman AH, Burgess WH, Tenner AJ. cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro. Immunity. 1997; 6(2):119-129. (Clone-specific). 참조 보기
Nepomuceno RR, Ruiz S, Park M, Tenner AJ. C1qRP is a heavily O-glycosylated cell surface protein involved in the regulation of phagocytic activity. J Immunol. 1999; 162(6):3583-3589. (Clone-specific). 참조 보기
Nepomuceno RR, Tenner AJ. C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets. J Immunol. 1998; 160(4):1929-1935. (Clone-specific). 참조 보기
Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 참조 보기
Tenner AJ. C1q receptors: regulating specific functions of phagocytic cells. Immunobiology. 1998; 199(2):250-264. (Biology). 참조 보기

모두 보기 (8)

552954 Rev. 3

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.