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- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Profile of CD68 expressed on permeabilized peripheral blood mononuclear cell (monocytes) analyzed on a FACScan (BDIS, San Jose, CA)
BD Pharmingen™ Purified Mouse Anti-Human CD68
규제 상태 범례
Becton, Dickinson and Company의 명시적인 서면 승인 없이는 사용 하실 수 없습니다.
준비 및 보관
권장 분석 절차
We recommend the use of Cytofix/Cytoperm (Cat. No. 554714) for the cells fixation and permeabilization, and then do the intracellular staining.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
The Y1/82A monoclonal antibody specifically binds to CD68 which is also known as, Scavenger receptor class D member 1 (SCARD1), Macrosialin, or GP110. CD68 is a cell surface 110 kDa-type I-transmembrane glycoprotein that is primarily expressed in cytoplasmic granules of monocytes, macrophages, dendritic cells, granulocytes, myeloid progenitor cells and, reportedly, a subset of CD34-positive hemopoietic bone marrow progenitor cells. CD68 belongs to the sialomucin family and serves as a scavenger receptor that can bind and internalize oxidized low density lipoproteins (LDL). This antibody is useful in studies of myeloid cell development and function.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
개발 참고 자료 (4)
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Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
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Davey FR, Cordell JL, Erber WN, Pulford KA, Gatter KC, Mason DY. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol. 1988; 41(7):753-758. (Clone-specific: Immunohistochemistry). 참조 보기
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Davey FR, Erber WN, Gatter KC, Mason DY. The use of monoclonal antibody Y1/82A in the identification of acute myeloblastic and monocytic leukemias. Am J Clin Pathol. 1988; 89(1):76-80. (Clone-specific: Immunohistochemistry). 참조 보기
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Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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