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      Purified Mouse Anti-Human CD57
      제품 세부 정보
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      BD Pharmingen™
      Hu CD57 Pure HNK-1 200ug
      Human (QC Testing)
      Mouse IgM, κ
      Membrane extracts of the HSB-2 T-lymphoblastoid cell line
      Flow cytometry (Routinely Tested), Functional assay, Immunohistochemistry-paraffin, Western blot (Reported)
      1.0 mg/ml
      AB_397184
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      준비 및 보관

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      권장 분석 절차

      Functional Studies: Anti-HNK-1 monoclonal antibody recognizes a subset of NK lymphocytes and T lymphocytes. Unlike the populations of cells recognized by Anti-N-CAM monoclonal antibodies that share the unique ability to mediate direct, non-major histocompatibility complex-restricted cytotoxicity against certain tumor cells, the T lymphocytes recognized by anti-HNK-1 monoclonal antibody do not have NK activity. Anti-HNK-1 monoclonal antibodies can be used with other anti-N-CAM monoclonal antibodies to better understand the role of HNK-1 and N-CAM in leukocyte cytolytic activity against tumor cells. Anti-HNK-1 monoclonal antibody can be used in studies on the role of N-CAM and HNK-1 in the development and regulation of neuroendocrine tissues and muscle regeneration.

      Immunohistology: Anti-HNK-1 monoclonal antibody can be used to stain paraffin-embedded tissues.

      Immunoblotting: Anti-HNK-1 monoclonal antibody can be used in Western blot analysis.

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      제품 고시

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      559048 Rev. 6
      항체 세부 정보
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      HNK-1

      Anti-HNK-1 monoclonal antibody recognizes a 110 kD glycoprotein (CD57) that is expressed on a subset of natural killer (NK) lymphocytes and T lymphocytes. It also recognizes epitopes on neural or neuroectodermal tumors including neurofibromas, malignant melanomas, malignant peripheral neuroectodermal tumors, Ewing's sarcomas, and small-cell lung carcinomas. Anti-HNK-1 monoclonal antibody also recognizes a carbohydrate epitope on subpopulations of several cell-adhesion molecules including N-CAM and myelin-associated glycoprotein (MAG).

      559048 Rev. 6
      형광 세부 정보
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      559048 Rev.6
      인용 및 참고 문헌
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      개발 참고 자료 (16)

      1. Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981; 127(3):1024-1029. (Biology). 참조 보기
      2. Bunn PA Jr, Linnoila I, Minna JD, Carney D, Gazdar AF.. Small cell lung cancer, endocrine cells of the fetal bronchus, and other neuroendocrine cells express the Leu-7 antigenic determinant present on natural killer cells.. Blood. 1985 March; 65(3):764-768. (Biology). 참조 보기
      3. Caillaud JM, Benjelloun S, Bosq J, Braham K, Lipinski M.. HNK-1-defined antigen detected in paraffin-embedded neuroectoderm tumors and those derived from cells of the amine precursor uptake and decarboxylation system.. Cancer Res. 1984 October; 44(10):4432-4439. (Biology). 참조 보기
      4. Hemperly JJ, Murray BA, Edelman GM, Cunningham BA. Sequence of a cDNA clone encoding the polysialic acid-rich and cytoplasmic domains of the neural cell adhesion molecule N-CAM. Proc Natl Acad Sci U S A. 1986; 83(9):3037-3041. (Biology). 참조 보기
      5. Jin L, Hemperly JJ, Lloyd RV. Expression of neural cell adhesion molecule in normal and neoplastic human neuroendocrine tissues. Am J Pathol. 1991; 138(4):961-969. (Biology). 참조 보기
      6. Kruse J, Mailhammer R, Wernecke H, et al. Neural cell adhesion molecules and myelin-associated glycoprotein share a common carbohydrate moiety recognized by monoclonal antibodies L2 and HNK-1.. Nature. 1984 September; 311(5982):153-155. (Biology). 참조 보기
      7. Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986; 136(12):4480-4486. (Biology). 참조 보기
      8. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). 참조 보기
      9. Lipinski M, Braham K, Caillaud JM, Carlu C, Tursz T.. HNK-1 antibody detects an antigen expressed on neuroectodermal cells.. J Exp Med. 1983 November; 158(5):1775-1780. (Biology). 참조 보기
      10. Llombart-Bosch A, Lacombe MJ, Peydro-Olaya A, Perez-Bacete M, Contesso G.. Malignant peripheral neuroectodermal tumours of bone other than Askin's neoplasm: characterization of 14 new cases with immunohistochemistry and electron microscopy.. Virchows Arch A Pathol Anat Histopathol. 1988; 412(5):421-430. (Biology). 참조 보기
      11. Manara GC, De Panfilis G, Ferrari C.. Ultrastructural characterization of human large granular lymphocyte subsets defined by the expression of HNK-1 (Leu-7), Leu-11, or both HNK-1 and Leu-11 antigens.. J Histochem Cytochem. 1985 November; 33(11):1129-1133. (Biology). 참조 보기
      12. Mechtersheimer G, Staudter M, Möller P.. Expression of the natural killer cell-associated antigens CD56 and CD57 in human neural and striated muscle cells and in their tumors.. Cancer Res. 1991 February; 51(4):1300-1307. (Biology). 참조 보기
      13. Pinto A, Grant LH, Hayes FA, Schell MJ, Parham DM.. Immunohistochemical expression of neuron-specific enolase and Leu 7 in Ewing's sarcoma of bone.. Cancer. 1989 September; 64(6):1266-1273. (Biology). 참조 보기
      14. Schubert W, Zimmermann K, Cramer M, Starzinski-Powitz A. Lymphocyte antigen Leu-19 as a molecular marker of regeneration in human skeletal muscle. Proc Natl Acad Sci U S A. 1989; 86(1):307-311. (Biology). 참조 보기
      15. Smolle J, Walter GF, Kerl H.. Myelin-associated glycoprotein in neurogenic tumours of the skin: an immunohistological study using Leu-7 monoclonal antibody.. Arch Dermatol Res. 1985; 277(2):141-142. (Biology). 참조 보기
      16. Swanson PE, Manivel JC, Wick MR.. Immunoreactivity for Leu-7 in neurofibrosarcoma and other spindle cell sarcomas of soft tissue.. Am J Pathol. 1987 March; 126(3):546-560. (Biology). 참조 보기
      모두 보기 (16) 간단히 보기
      559048 Rev. 6

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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