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Upregulation of H2-M3 expression by exogenous N-formylated peptide. C57BL/6 splenocytes were cultured overnight in the absence (left panel) or presence (right panel) of N-Formyl-Met-Leu-Phe-Phe peptide (Sigma-Aldrich). The cells were then stained with either purified hamster anti- mouse H2-M3 mAb (clone 130) (open histograms) or purified hamster IgG1, κ isotype control mAb (clone A19-3) (Cat. No. 553969, filled histograms), in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32, mAb (clone 2.4G2) (Cat. No. 553141, open and filled histograms), followed by PE mouse anti-hamster IgG cocktail (Cat. No. 554056, open and filled histograms). Flow cytometry was performed on a BD FACSCalibur™ instrument.
BD Pharmingen™ Purified Hamster Anti-Mouse H2-M3
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제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
The hamster anti-mouse H2-M3 antibody (clone 130) reacts with the H2-M3 major histocompatibility complex (MHC) non-classical class Ib antigen. H2-M3 (M3) associates with β2-microglobulin and is capable of being expressed by most leukocytes. However, due to a lack of endogenous antigens, M3 has been reported to be undetectable on most cells. Its expression is induced by high-affinity N-formylated peptides from mitochondria, Listeria monocytogenes, and Mycobacterium tuberculosis. The induced expression of M3 is most efficient on antigen presenting cells. M3 presents antigen to cytotoxic T lymphocytes and may play a key role in protective immunity against the intracellular bacteria L. monocytogenes and M. tuberculosis. Furthermore, M3 is capable of presenting mitochondrial antigens for intrathymic positive selection of T-cell receptors which recognize those peptides.
개발 참고 자료 (6)
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Berg RE, Princiotta MF, Irion S, Moticka JA, Dahl KR, Staerz UD. Positive selection of an H2-M3 restricted T cell receptor. Immunity. 1999; 11(1):33-43. (Biology). 참조 보기
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Chiu NM, Chun T, Fay M, Mandal M, Wang CR. The majority of H2-M3 is retained intracellularly in a peptide-receptive state and traffics to the cell surface in the presence of N-formylated peptides. J Exp Med. 1999; 190(3):423-434. (Immunogen: Immunoprecipitation). 참조 보기
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Chun T, Serbina NV, Nolt D, et al. Induction of M3-restricted cytotoxic T lymphocyte responses by N-formylated peptides derived from Mycobacterium tuberculosis. J Exp Med. 2001; 193(10):1213-1220. (Biology). 참조 보기
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Lindahl KF, Byers DE, Dabhi VM, et al. H2-M3, a full-service class Ib histocompatibility antigen. Annu Rev Immunol. 1997; 15:851-879. (Biology). 참조 보기
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Seaman MS, Perarnau B, Lindahl KF, Lemonnier FA, Forman J. Response to Listeria monocytogenes in mice lacking MHC class Ia molecules. J Immunol. 1999; 162(9):5429-5436. (Biology). 참조 보기
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Shawar SM, Vyas JM, Rodgers JR, Rich RR. Antigen presentation by major histocompatibility complex class I-B molecules. Annu Rev Immunol. 1994; 12:839-880. (Biology). 참조 보기
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