BD Pharmingen™ PE-Texas Red Streptavidin

PE-Texas Red is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by the 488-nm light of an Argon ion laser and serves as an energy donor, coupled to the Texas Red™ molecule, which acts as the energy acceptor and fluoresces at 615 nm.

SAv-PE-TxR is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis.  

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
4. Single-laser cytometers equipped with 650- or 670-nm long-pass filters such as the BD FACScan™ and BD FACSCalibur™ Flow Cytometry Systems are not optimal for the detection of PE-Texas Red and will require higher compensation for FL2 - FL3.
5. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers with dye lasers, which may directly excite both PE and Texas Red®. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis. We recommend that a 610/20-nm band-pass filter be used for optimum fluorescence detection of PE-Texas Red emission.
6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.