Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Multicolor flow cytometric analysis of Zbtb20 expression in B1 or B2 subsets of mouse peritoneal B cells. Peritoneal cells were stained with APC-Cy™7 Rat Anti-Mouse CD45R/B220 (Cat. No. 552094), BD Horizon™ BV421 Rat Anti-Mouse IgM (Cat. No. 562595 ), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD23 (Cat. No. 563986) antibodies. The cells were then washed, fixed, and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 554689; dashed line histograms) or PE Rat Anti-Mouse Zbtb20 antibody (Cat. No. 565454; solid line histograms). The fluorescence histograms showing Zbtb20 expression (or Ig Isotype control staining) were derived from either B220+ IgM+ CD23- (B1 cells; Top Right Plot) or B220+ IgM low CD23+ (B2 cells; Bottom Right Plot) gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
BD Pharmingen™ PE Rat Anti-Mouse Zbtb20
규제 상태 범례
Becton, Dickinson and Company의 명시적인 서면 승인 없이는 사용 하실 수 없습니다.
준비 및 보관
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The 4A3 monoclonal antibody specifically binds to Zinc finger and BTB domain containing 20 (Zbtb20). This transcription factor is also known as Zinc finger protein 288 (Zfp288), BTB/POZ domain zinc finger factor HOF, BTB/POZ zinc finger protein DPZF, or POZ/zinc finger transcription factor ODA-8. Zbtb20 is a transcription factor that localizes to the nucleus and contains one BTB/POZ domain and 5 C2H2-type zinc fingers. Mouse ZBTB20 is expressed in thymus, spleen, lymph node, peritoneal cells and fetal liver. It is highly expressed in B1 and germinal center B cells, and long-lived antibody-secreting plasma cells. In cell lines, Zbtb20 is expressed at low levels in the immature WEHI231 and the mature 129B cell lines but at high levels in A20 cells, a germinal center B cell lymphoma. This transcription factor reportedly plays key roles in hematopoiesis, immunity, and oncogenesis.
개발 참고 자료 (2)
-
Chevrier S, Emslie D, Shi W, et al. The BTB-ZF transcription factor Zbtb20 is driven by Irf4 to promote plasma cell differentiation and longevity. J Exp Med. 2014; 211(5):827-840. (Immunogen: Flow cytometry, Western blot). 참조 보기
-
Xie Z, Ma X, Ji W, et al. Zbtb20 is essential for the specification of CA1 field identity in the developing hippocampus. Proc Natl Acad Sci U S A. 2010; 107(14):6510-6515. (Biology). 참조 보기
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.