-
Reagents
- Flow Cytometry Reagents
-
Western Blotting and Molecular Reagents
- Immunoassay Reagents
-
Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
-
Functional Assays
-
Microscopy and Imaging Reagents
-
Cell Preparation and Separation Reagents
-
- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- Korea (Korea)
- 국가 / 언어 변경
Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Multicolor flow cytometric analysis of P2X7 receptor expression on mouse cells. Left Panel: C57BL/6 mouse peritoneal cells were stained with BD Horizon™ BV421 Rat Anti-Mouse F4/80 (Cat. No. 565411) and APC Rat Anti-Mouse CD117 (Cat. No. 553356) antibodies, and either PE Rat IgG2b, κ Isotype Control (Cat. No. 556925; dashed line histogram) or PE Rat Anti-Mouse P2X7 antibody (Cat. No. 565345; solid line histogram). The fluorescence histogram showing P2X7 expression (or Ig Isotype control staining) was derived from F4/80- CD117+ gated events with the forward and side light-scatter characteristics of viable cells. Right Panel: Mouse splenocytes were stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 (Cat. No. 562922; Left Plots) and FITC Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 555274/561798; Right Plots) antibodies, and either PE Rat IgG2b, κ Isotype Control or PE Rat Anti-Mouse P2X7 antibody as indicated. Two-color flow cytometric dot plots showing the correlated expression of CD45R/B220 or CD3 versus P2X7 (or Ig Isotype control staining) were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
BD Pharmingen™ PE Rat Anti-Mouse P2X7
규제 상태 범례
Becton, Dickinson and Company의 명시적인 서면 승인 없이는 사용 하실 수 없습니다.
준비 및 보관
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The 1F11monoclonal antibody specifically binds to the P2X7 receptor. P2X7 is also known as the P2X7 Receptor (P2X7R), Purinergic receptor P2X ligand-gated ion channel 7 (P2RX7), P2X7 purinoceptor, P2Z receptor, or ATP receptor. P2X7 is differentially expressed on a wide variety of cell types including T cells, B cells, macrophages, dendritic cells, mast cells, neurons, microglia, and astrocytes. Functional P2X receptors exist as trimers. Each P2X7 monomer contains intracellular N and C termini, two transmembrane domains, and a large extra cellular loop. When present at relatively high levels, extracellular adenosine 5'-triphosphate (ATP) binds to the P2X7 receptor and opens a channel for extracellular cation (eg, Na+, K+, Ca++) influx into cells. The P2X7 receptor-mediated cation influx can cause membrane depolarization and Ca++ mediated signaling cascades that trigger cellular responses, eg, the production and/or release of proinflammatory mediators (eg, IL-1β or IL-18) by cells of the immune system. P2X7 is also implicated in mediating ATP-induced apoptosis of cells. The 1F11 antibody reportedly blocks ATP-mediated cellular activation through P2X7R.
개발 참고 자료 (4)
-
Bulanova E, Bulfone-Paus S. P2 receptor-mediated signaling in mast cell biology. Purinergic Signal. 2010; 6(1):3-17. (Biology). 참조 보기
-
Cloning and functional characterisation of the mouse P2X7 receptor. Cloning and functional characterisation of the mouse P2X7 receptor. FEBS Lett. 1998; 439(1-2):26-30. (Biology). 참조 보기
-
Idzko M, Ferrari D, Eltzschig HK. Nucleotide signalling during inflammation. Nature. 2014; 509(7500):310-317. (Biology).
-
Kurashima Y, Amiya T, Nochi T, et al. Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors.. Nat Commun. 2012; 3:1034. (Immunogen: Functional assay, Immunoprecipitation, Inhibition, In vivo exacerbation). 참조 보기
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.