-
Reagents
- Flow Cytometry Reagents
-
Western Blotting and Molecular Reagents
- Immunoassay Reagents
-
Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
-
Functional Assays
-
Microscopy and Imaging Reagents
-
Cell Preparation and Separation Reagents
-
- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- Korea (Korea)
- 국가 / 언어 변경
Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Flow cytometric analysis of Ly-6D expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were simultaneously stained with PE Rat Anti-Mouse Ly-6D (Cat. No. 561149; left panel) or PE Rat IgG2c Isotype Control (Cat. No. 559841; right panel) and PerCP-Cy™5.5 Anti-Mouse CD3 (Cat. No. 561108). A two-color flow cytometric dot plot showing the correlated expression patterns of Ly-6D versus CD3 was derived from gated events with the forward and side light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD LSR™ II.
BD Pharmingen™ PE Rat Anti-Mouse Ly-6D
규제 상태 범례
Becton, Dickinson and Company의 명시적인 서면 승인 없이는 사용 하실 수 없습니다.
준비 및 보관
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The 49-H4 antibody specifically binds to the ThB antigen (Ly-6D), a 15-kDa GPI-anchored protein of the Ly-6 Multigene Family. ThB has been detected on cortical thymocytes, small sIg- Thy-1- and sIg+ lymphoid cells in the bone marrow, thymic medullary epithelial cells, all epidermal layers (except the stratum corneum), and on peripheral B lymphocytes (but not peripheral T lymphocytes). There is strain-to-strain variation in ThB antigen expression on splenic B cells. Strains with the Ly-6.2 haplotype (e.g., AKR, C57BL, DBA/2, SJL, SWR) stain more intensely with anti-ThB reagents than Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, NZB); B cells of Ly-6.1 and Ly-6.2 hybrids stain with intermediate intensity. The proportion of ThB+ B cells to thymocytes do not differ significantly among strains.
개발 참고 자료 (6)
-
Boyd RL, Tucek CL, Godfrey DI. The thymic microenvironment. Immunol Today. 1993; 14(9):445-459. (Biology). 참조 보기
-
Eckhardt LA, Herzenberg LA. Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression. Immunogenetics. 1980; 11(3):275-291. (Clone-specific: Cytotoxicity). 참조 보기
-
Godfrey DI, Izon DJ, Tucek CL, Wilson TJ, Boyd RL. The phenotypic heterogeneity of mouse thymic stromal cells. Immunology. 1990; 70(1):66-74. (Biology). 참조 보기
-
Gumley TP, McKenzie IF, Kozak CA, Sandrin MS. Isolation and characterization of cDNA clones for the mouse thymocyte B cell antigen (ThB). J Immunol. 1992; 149(8):2615-2618. (Biology). 참조 보기
-
Gumley TP, McKenzie IF, Sandrin MS. Polymorphism at the mouse Thb locus. Immunogenetics. 1994; 39(6):390-394. (Clone-specific: Immunoprecipitation). 참조 보기
-
Matossian-Rogers A, Rogers P, Ledbetter JA, Herzenberg LA. Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6. Immunogenetics. 1982; 15(6):591-599. (Clone-specific: Immunoprecipitation). 참조 보기
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.