PE Rat anti-Mouse FR4 (Folate receptor 4)

BD Pharmingen™ PE Rat anti-Mouse FR4 (Folate receptor 4)

Name: PE Rat anti-Mouse FR4 (Folate receptor 4)
Brand: BD Pharmingen™
SKU: 560320

Clone 12A5

(RUO)

Flow cytometric analysis of PE anti-mouse FR4 on mouse splenocytes. Splenocytes from BALB/c mice were stained simultaneously with FITC Rat Anti-Mouse CD25 (clone 7D4, Cat. No. 553072), APC Rat Anti-Mouse CD4 (clone RM4-5, Cat. No. 553051) and PE Rat anti-Mouse FR4. The left panel shows the gated lymphocyte populations used to generate the histograms in the right panel. The histogram shows expression of FR4 as CD4+CD25+ gated lymphocytes (solid line) and CD25- gated lymphocytes (dashed line). Flow cytometry was performed on a BD FACSCalibur™ System

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제품 세부 정보

Brand: BD Pharmingen™

Product Name: Ms FR4 PE 12A5 100ug

Alternative Name: Folate receptor 4, FBP, FBP3, FRdelta, FRd

Reactivity: Mouse (QC Testing)

Isotype: Rat WI, also known as Wistar (outbred) IgG1

Immunogen: Mouse CD4+ CD25+ T cells

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 64931

RRID: AB_1645253

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

관련 제품

PE Rat IgG1, κ Isotype Control RUO

사이즈 0.1 mg 카탈로그 No 553925

FITC Rat Anti-Mouse CD25 RUO

사이즈 0.5 mg 카탈로그 No 553072

APC Rat Anti-Mouse CD4 RUO

사이즈 0.1 mg 카탈로그 No 553051

560320 Rev. 1

항체 세부 정보

12A5

The monoclonal antibodies TH6 and 12A5 recognize Folate Receptor 4 (FR4), also known as the membrane folate-binding protein 3 (FBP3). FR4 is a heavily glycosylated 35 kD receptor expressed exclusively in lymphoid tissue and an isoform of the family of receptors that recognize the essential nutrient folic acid. Natural T regulatory cells constitutively express high levels of FR4. Differential expression of FR4 in combination with CD25 can distinguish four functionally distinct CD4+ T cell subpopulations; Natural Tregs, effector T cells, memory-like T cells and Naïve T cells. FR4hi CD25+ expressing CD4+ T cells also express high amounts of Foxp3, GITR and CTLA-4.

Monoclonal antibody TH6 and 12A5 stained CD25+CD4+ T cells at a higher level than other CD4+ or CD8+ T cells. In addition, in vivo injection of TH6 monoclonal antibody reduced the number of CD25+CD4+ T cells and CD25-CD4+ T cells in peripheral blood. Clone 12A5 has been demonstrated to work in western blot. Clones TH6 and 12A5 do not block one another in a flow cytometric assay.

560320 Rev. 1

형광 세부 정보

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

560320 Rev.1

인용 및 참고 문헌

개발 참고 자료 (3)

Rothberg KG, Ying YS, Kolhouse JF, Kamen BA, Anderson RG. The glycophospholipid-linked folate receptor internalizes folate without entering the clathrin-coated pit endocytic pathway. J Cell Biol. 1990; 110(3):637-649. (Biology). 참조 보기
Spiegelstein O, Eudy JD, Finnell RH. Identification of two putative novel folate receptor genes in humans and mouse. Gene. 2000; 258(1-2):117-125. (Biology). 참조 보기
Yamaguchi T, Hirota K, Nagahama K, et al. Control of immune responses by antigen-specific regulatory T cells expressing the folate receptor.. Immunity. 2007; 27(1):145-59. (Biology). 참조 보기

560320 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.