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Multicolor flow cytometric analysis of mouse CD370 (Clec9A) expression in spleen cells. BALB/c splenocytes were simultaneously stained with Alexa Fluor® 700 Rat Anti-Mouse CD4 (Cat. No. 557956), APC Rat Anti-Mouse CD8 (Cat. No. 553035), FITC Hamster Anti-Mouse CD11c (Cat. No. 553801) antibodies and either PE Rat IgG2a κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD370 (Clec9A) antibody (Cat. No. 562734; RIght Plot). Two color flow cytometric dot blots showing the correlated expression pattern of CD11c and Clec9A (or Ig Isotype control staining) were generated from CD8+ CD4- gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ PE Rat anti-Mouse CD370 (Clec9A)
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준비 및 보관
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The 10B4 monoclonal antibody specifically binds to mouse Clec9A. Mouse Clec9A (C-type lectin domain family member 9A) is also known as DNGR1 (Dendritic cell natural killer lectin group receptor 1). It is a type II membrane protein with a single extracellular C-type lectin domain. Clec9A is a dendritic cell subtype-restricted C-type lectin-like receptor. Clec9A is selectively expressed on plasmacytoid dendritic cells and CD8+ myeloid dendritic cells. Clec9A reportedly serves as a receptor for necrotic cells. It can mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
개발 참고 자료 (3)
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Caminschi L, Proietto AL, Ahmet F, et al. The dendritic cell subtype restricted C-type lectin Clec9A is a target for vaccine enhancement. Blood. 2008; 112(8):3264-3273. (Immunogen). 참조 보기
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Huysamen C, Willment JA, Dennehy KM, Brown GD. CLEC9A is a novel activation C-type lectin-like receptor expressed on BDCA3+ dendritic cells and a subset of monocytes. J Biol Chem. 2008; 283(24):16693-16701. (Biology). 참조 보기
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Sancho D, Joffre OP, Keller AM, et al. Identification of a dendritic cell receptor that couples sensing of necrosis to immunity. Nature. 2009; 458(7240):899-903. (Biology). 참조 보기
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