Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Multi-parameter flow cytometric analysis of CD181 (CXCR1) expression on mouse blood neutrophils (left and middle panel). Mouse blood cells were stained simultaneously with APC Rat Anti-Mouse Ly-6G antibody (Cat. No. 560599), BD Horizon™ BV421 Rat Anti-Mouse CD11b antibody (Cat. No. 562605), and either PE Rat IgG2b, κ Isotype Control (Cat. No. 555848) or PE Rat Anti-Mouse CD181 (CXCR1) antibody (Cat. No. 566383) at 0.5 µg/test. Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The left panel displays two-color flow cytometric plots showing the correlated expression patterns of CD11b versus Ly-6G from gated events with the forward and side light-scatter characteristics of viable cells. The middle panel displays the fluorescence histograms of CD181 (CXCR1) expression (solid line histogram) or Ig Isotype control staining (dashed line histogram) on gated Ly-6G and CD11b double-positive neutrophils. Blood leukocytes that were Ly-6G and CD11b double-negative did not express CD181 (data not shown). Flow cytometric analysis of CD181 (CXCR1) expression on transfected 293F cells (right panel). Untransfected (dashed line histogram) and mouse CD181-transfected (solid line histogram) 293F cells were stained with PE Rat Anti-Mouse CD181 (CXCR1) antibody (Cat.No. 566383) at 0.5 ug/test. The fluorescence histogram showing CD181 (CXCR1) expression was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed on a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Rat Anti-Mouse CD181 (CXCR1)
규제 상태 범례
Becton, Dickinson and Company의 명시적인 서면 승인 없이는 사용 하실 수 없습니다.
준비 및 보관
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The U45-632 monoclonal antibody specifically binds to mouse CD181, also known as C-X-C chemokine receptor type 1 (CXCR1) or IL-8 receptor α. CD181 is a chemokine receptor of the G-protein coupled receptor 1 family, also known as seven-transmembrane domain receptors. In the mouse, which is not known to express IL-8 (CXCL8), CD181 binds to mouse GCP-2 (CXCL6). Mouse CD181 can also bind to human IL-8. CD181 is expressed by neutrophils, which are activated upon ligand binding. The chemokine receptors CXCR1 and CXCR2 play an important role in mediating neutrophil recruitment and neutrophil-dependent injury in several models of inflammation.
개발 참고 자료 (4)
-
Fan X, Patera AC, Pong-Kennedy A, et al. Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8.. J Biol Chem. 2007; 282(16):11658-66. (Biology). 참조 보기
-
Fu W, Zhang Y, Zhang J, Chen WF. Cloning and characterization of mouse homolog of the CXC chemokine receptor CXCR1.. Cytokine. 2005; 31(1):9-17. (Biology). 참조 보기
-
Min SH, Wang Y, Gonsiorek W, et al. Pharmacological targeting reveals distinct roles for CXCR2/CXCR1 and CCR2 in a mouse model of arthritis.. Biochem Biophys Res Commun. 2010; 391(1):1080-6. (Biology). 참조 보기
-
Moepps B, Nuesseler E, Braun M, Gierschik P. A homolog of the human chemokine receptor CXCR1 is expressed in the mouse.. Mol Immunol. 2006; 43(7):897-914. (Biology). 참조 보기
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.