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Profile of LPS-stimulated (24 hrs) peripheral blood monocytes of rhesus macaque monkey (Macaca mulatta) analyzed by flow cytometry
BD Pharmingen™ PE Rat Anti-Human IL-6
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제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
IL-6, a 26 kDa protein, is a multifunctional cytokine secreted by both lymphoid and non-lymphoid cells. It regulates B and T cell function and hematopoiesis. The MQ2-6A3 antibody reacts with human interleukin-6 (IL-6). The immunogen used to generate the MQ2-6A3 hybridoma was recombinant human IL-6. This is a neutralizing antibody. Clone MQ2-6A3 cross-reacts with an intracellular component of peripheral blood LPS-stimulated permeabilized monocytes of rhesus and cynomolgus macaques. The reactivity pattern observed on non-human primates is similar, although weaker, than that seen on normal human monocytes.
개발 참고 자료 (2)
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Villinger F, Brar SS, Mayne A, Chikkala N, Ansari AA. Comparative sequence analysis of cytokine genes from human and nonhuman primates. J Immunol. 1995; 155(8):3946-3954. (Biology). 참조 보기
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Villinger F, Hunt D, Mayne A, Vuchetich M, Findley H, Ansari AA. Qualitative and quantitative studies of cytokines synthesized and secreted by non-human primate peripheral blood mononuclear cells. Cytokine. 1993; 5(5):469-479. (Biology). 참조 보기
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