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제품 세부 정보
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BD Pharmingen™
Il17f; interleukin 17F; Interleukin-17F
Mouse (QC Testing)
Mouse BALB/c IgG1, κ
Mouse IL-17F Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_10716055
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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제품 고시

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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561627 Rev. 1
항체 세부 정보
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O79-289

The O79-289 monoclonal antibody specifically binds to the inflammatory cytokine protein, Interleukin-17 (IL-17F). IL-17F is a member of the IL-17 family of cytokines. Among IL-17 family members, IL-17F has the highest amino acid sequence homology to IL-17A. IL-17F is produced by activated CD4+ T helper (Th17) cells, CD8+ T (Tc17) cells and γδ T cells. IL-17F can be secreted as homodimers or as heterodimers with IL-17A. IL-17F and IL-17A have overlapping functions such as inducing epithelial cells and fibroblasts to produce proinflammatory cytokines and chemokines including IL-6, GM-CSF, CXCL1, CCL2, and CCL7. These factors attract and activate neutrophils and other cell types that mediate protective responses against pathogenic microbes or pathologic allergic or autoimmune diseases. IL-17 gene knockout studies have shown that IL-17F and IL-17A have independent functions as well. IL-17F and IL-17A exert their biological function by binding to and signaling through IL-17 receptors comprised of the transmembrane receptor subunits, IL-17RA (CD217) and IL-17RC.

561627 Rev. 1
형광 세부 정보
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
496 nm, 566 nm
576 nm
561627 Rev.1
인용 및 참고 문헌
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View product citations for antibody "561627" on CiteAb

개발 참고 자료 (8)

  1. Chang SH, Dong C. IL-17F: Regulation, signaling and function in inflammation. Cytokine. 2009; 46(1):7-11. (Biology). 참조 보기
  2. Dong C. Th17 cells: Current understanding of their generation and regulation. Eur J Immunol. 2009; 39(3):640-644. (Biology: Cell culture, Cell differentiation). 참조 보기
  3. Hamada H, Garcia-Hernandez MdlL, Reome JB, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009; 182(6):3469-3481. (Biology). 참조 보기
  4. Ishigame H, Kakuta S, Nagai T, et al. Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses. Immunity. 2009; 30(1):108-119. (Biology). 참조 보기
  5. Martinez GJ, Zhang Z, Chung Y, et al. Smad3 differentially regulates the induction of regulatory and inflammatory T cell differentiation. J Biol Chem. 2009; 284(51):35283-35286. (Biology: Cell culture, Cell differentiation). 참조 보기
  6. Oda N, Canelos PB, Essayan DM, Plunkett BA, Myers AC, Huang SK. Interleukin-17F induces pulmonary neutrophilia and amplifies antigen-induced allergic response. Am J Respir Crit Care Med. 2005; 171(1):12-13. (Biology). 참조 보기
  7. Sutton CE, Lalor SJ, Sweeney CM, Brereton CF, Lavelle EC, Mills KH. Interleukin-1 and IL-23 induce innate IL-17 production from gammadelta T cells, amplifying Th17 responses and autoimmunity. Immunity. 2009; 31(2):331-341. (Biology). 참조 보기
  8. Yang XO, Chang SH, Park H, et al. Regulation of inflammatory responses by IL-17F. J Exp Med. 2008; 205(5):1063-1075. (Biology). 참조 보기
모두 보기 (8) 간단히 보기
561627 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.