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Profile of TWEAK (CD255) reactivity on TWEAK transfectant cell line analyzed by flow cytometry
BD Pharmingen™ PE Mouse Anti-Human TWEAK (CD255)
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준비 및 보관
제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
The CARL-2 monoclonal antibody specifically binds to TWEAK (TNF-related WEAK inducer of apoptosis), a type II transmembrane protein of approximately 1.4 kDa. TWEAK is also known as CD255. It is present as a cell surface bound or a secreted form, expressed on most tissues and non-lymphoid tumor cell lines, but weak or no expression on hematopoietic lineage lines. TWEAK is not detectable on resting PBMC, but its expression on peripheral blood monocytes is upregulated upon IFN-g stimulation. Reports indicate that TWEAK interacts with its receptor APO-3 and this interaction can lead to apoptosis, IL-8 secretion, NF-kB activation and endothelial cell proliferation. Clone CARL-1, a different monoclonal antibody, also reacts with CD255 (TWEAK) but to a different epitope.
개발 참고 자료 (4)
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Chicheportiche Y, Bourdon PR, Xu H, et al. TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J Biol Chem. 1997; 272(51):32401-32410. (Biology). 참조 보기
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Kaplan MJ, Ray D, Mo RR, Yung RL, Richardson BC. TRAIL (Apo2 ligand) and TWEAK (Apo3 ligand) mediate CD4+ T cell killing of antigen-presenting macrophages. J Immunol. 2000; 164(6):2897-2904. (Biology). 참조 보기
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Lynch CN, Wang YC, Lund JK, Chen YW, Leal JA, Wiley SR. TWEAK induces angiogenesis and proliferation of endothelial cells. J Biol Chem. 1999; 274(13):8455-8459. (Biology). 참조 보기
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Nakayama M, Kayagaki N, Yamaguchi N, Okumura K, Yagita H. Involvement of TWEAK in interferon gamma-stimulated monocyte cytotoxicity. J Exp Med. 2000; 192(9):1373-1380. (Clone-specific: Blocking, Flow cytometry, Functional assay, Inhibition). 참조 보기
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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