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Flow cytometric analysis of human LDLR expression. Untransfected (dashed line histogram) and human LDLR-transfected (solid line histogram) 293F cells were washed and stained with PE Mouse Anti-Human LDLR (Cat. No. 565653). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto™ II cell analyzer.
BD Pharmingen™ PE Mouse Anti-Human LDLR
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제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.
관련 제품
The C7 monoclonal antibody specifically binds to the low-density lipoprotein (LDL) receptor, a type I membrane protein that is encoded by the LDLR gene. LDL is the major cholesterol-carrying lipoprotein in plasma. Cell surface LDLR controls the level of cholesterol in plasma by binding to and internalizing LDL and transporting it to lysosomes where LDL is degraded, cholesterol is released into the cell, and LDLR is recycled back to the cell surface. Hence LDLR is found in cell-surface and intracellular membranes (eg, clathrin-coated pits, golgi, endosomes, and lysosomes). Expression of LDLR is a marker for in vitro differentiation of hepatocytes from human embryonic stem cells. LDLR is suspected to mediate infections by viruses that associate with lipoprotein in the blood. Mutations in LDLR are largely responsible for Familial Hypercholesterolemia (FH). The C7 monoclonal antibody has been reported to react with bovine and human LDLR, but not LDLRs of mouse, rat, Chinese hamster, rabbit or dog.
개발 참고 자료 (6)
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Beisiegel U, Schneider WJ, Brown MS, Goldstein JL. Immunoblot analysis of low density lipoprotein receptors in fibroblasts from subjects with familial hypercholesterolemia. J Biol Chem. 1982; 257:13150-13156. (Clone-specific: Western blot). 참조 보기
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Beisiegel U, Schneider WJ, Goldstein JL, Anderson RG, Brown MS. Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J Biol Chem. 1981; 256(22):11923-11931. (Immunogen). 참조 보기
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Francke U, Brown MS, Goldstein JL. Assignment of the human gene for the low density lipoprotein receptor to chromosome 19: synteny of a receptor, a ligand, and a genetic disease. Proc Natl Acad Sci U S A. 1984; 81(9):2826-2830. (Clone-specific: Immunoprecipitation). 참조 보기
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Schneider WJ, Beisiegel U, Goldstein JL, Brown MS. Purification of the low density lipoprotein receptor, an acidic glycoprotein of 164,000 molecular weight. J Biol Chem. 1982; 257:2664-26673. (Clone-specific: Immunoaffinity chromatography). 참조 보기
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Touboul T, Hannan NR, Corbineau S, et al. Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development. Hepatology. 2010; 51(5):1754-1765. (Biology). 참조 보기
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Yamamoto T, Davis CG, Brown MS, et al. The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 1984; 39(1):27-38. (Biology). 참조 보기
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