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Multiparameter flow cytometric analysis of HLA-C expression on human peripheral blood leucocyte populations. The erythrocytes from human blood were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). After washing, the remaining leucocytes were stained with either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058, Left Plot) or PE Mouse Anti-Human HLA-C antibody (Cat. No. 566372; Right Plot). Two-parameter flow cytometric contour plots showing the correlated expression of HLA-C (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
BD Pharmingen™ PE Mouse Anti-Human HLA-C
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준비 및 보관
제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The DT-9 monoclonal antibody specifically recognizes Human Leukocyte Antigen (HLA-C), a polymorphic major histocompatibility complex (MHC) class I antigen. HLA-C is a heterodimer comprised of the HLA-C alpha chain, a ~45 kDa type I transmembrane glycoprotein, and a ~12 kDa Beta-2 (β2)-microglobulin light chain. HLA-C is expressed on nearly all cells, and plays a role in the antigen-specific, MHC-restricted presentation of small peptides to CD8+ T cells in the generation of immunity or tolerance. HLA-C may also bind to regulatory MHC class I antigen-selective receptors expressed by CD8+ T cells and natural killer (NK) cells. The DT-9 antibody also recognizes some rare HLA-A and HLA-B allotypes and the nonclassical HLA-E molecule.
개발 참고 자료 (6)
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Apps R, Qi Y, Carlson JM, et al. Influence of HLA-C expression level on HIV control.. Science. 2013; 340(6128):87-91. (Clone-specific: Flow cytometry). 참조 보기
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Braud VM, Allan DS, O'Callaghan CA, et al. HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C.. Nature. 1998; 391(6669):795-9. (Clone-specific: Flow cytometry). 참조 보기
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Braud VM, Allan DS, Wilson D, McMichael AJ. TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide.. Curr Biol. 1998; 8(1):1-10. (Immunogen: Flow cytometry, Immunoprecipitation). 참조 보기
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Corrah TW, Goonetilleke N, Kopycinski J, et al. Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression.. J Virol. 2011; 85(7):3367-74. (Clone-specific: Flow cytometry). 참조 보기
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Thomas R, Apps R, Qi Y, et al. HLA-C cell surface expression and control of HIV/AIDS correlate with a variant upstream of HLA-C.. Nat Genet. 2009; 41(12):1290-4. (Clone-specific: Flow cytometry). 참조 보기
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Zipeto D, Beretta A. HLA-C and HIV-1: friends or foes?. Retrovirology. 2012; 9:39. (Biology). 참조 보기
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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