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Flow cytometric analysis of PE anti-human HLA-A,B,C on lymphocytes. Whole blood was stained with PE anti-human HLA-A,B,C (clone DX17 , Cat. No. 560168) and compared to whole blood stained with a PE mouse IgG1 isotype control (clone MOPC-21, Cat. No. 555749). The isotype control is represented by a dashed line and the PE anti-human HLA-A,B,C by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable cells.
BD Pharmingen™ PE Mouse anti-Human HLA-ABC
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- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The DX17 monoclonal antibody reacts with a monomorphic epitope expressed on all HLA (human leukocyte antigen) class I molecules examined. DX17 immunoprecipitates HLA class I heavy chains (45 kDa) and β2-microglobulin (12kDa) from radiolabeled human cell lines. HLA is determined by a complex segment of the short arm of chromosome 6 and there are many human HLAs encoded in this segment. The antigenic agglomerate is called MHC, for major histocompatibility complex. Examples of class I loci are HLA-A, -B, and -C, which are serologically assayed; class II loci, e.g., HLA-D/DR and DC1, are tested by lymphocytotoxic methods.
개발 참고 자료 (4)
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Bach FH, Amos DB. Hu-1: Major histocompatibility locus in man. Science. 1967; 156(781):1506-1508. (Biology). 참조 보기
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Gumperz JE, Litwin V, Phillips JH, Lanier LL, Parham P. The Bw4 public epitope of HLA-B molecules confers reactivity with natural killer cell clones that express NKB1, a putative HLA receptor. J Exp Med. 1995; 181(3):1133-1144. (Biology). 참조 보기
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Lanier LL, Gumperz JE, Parham P, Melero I, López-Botet M, Phillips JH. The NKB1 and HP-3E4 NK cells receptors are structurally distinct glycoproteins and independently recognize polymorphic HLA-B and HLA-C molecules. J Immunol. 1995; 154(7):3320-3327. (Clone-specific). 참조 보기
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Morton CC, Kirsch IR, Nance WE, Evans GA, Korman AJ, Strominger JL. Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization. Proc Natl Acad Sci U S A. 1984; 81(9):2816-2820. (Biology). 참조 보기
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