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Flow cytometric analysis of FoxA2 in definitive endoderm derived from human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days (D'Amour et al, 2005) in RPMI medium supplemented with 0.5% FBS, 1x L-glutamine, and 100 ng/ml Activin A (R&D Systems). Control ES cells (left panel) and day-3 differentiated cells (right panel) were fixed with BD Cytofix buffer (Cat. No. 554655) and permeabilized with BD™ Phosflow Perm buffer III (Cat. No. 558050). The cells were stained with either PE Mouse IgG1, κ isotype control (dashed lines, Cat. No.554680) or PE Mouse Anti-human FoxA2 antibody (solid lines) at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSR™ II flow cytometry system. This antibody can also be used on fixed cells permeabilized with 0.1% Triton X-100.
BD Pharmingen™ PE Mouse anti-Human FoxA2
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제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
FoxA2, forkhead box A2, is a member of the forkhead class of DNA-binding proteins that regulates gene expression in the liver, pancreatic islets, adipocytes and some neural cells. This hepatocyte nuclear factor is a transcriptional activator for liver-specific genes such as alpha fetoprotein, albumin, tyrosine aminotransferase and transthyretin. FoxA2 is expressed in embryonic endoderm, the germ layer that gives rise to the digestive system, and contributes to the specification of the pancreas and the regulation of glucose homoeostasis. FoxA2 also has roles in neural development. Specifically, FoxA2 cooperates with related FoxA1 in the specification and differentiation of midbrain dopaminergic neurons in a dosage-dependent manner.
개발 참고 자료 (7)
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Burtscher I, Lickert H.. Foxa2 regulates polarity and epithelialization in the endoderm germ layer of the mouse embryo. Development. 2009; 136(6):1029-1038. (Biology). 참조 보기
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Clevidence DE, Overdier DG, Tao W, et al. Identification of nine tissue-specific transcription factors of the hepatocyte nuclear factor 3/forkhead DNA-binding-domain family. Proc Natl Acad Sci U S A. 1993; 90(9):3948-3952. (Biology). 참조 보기
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D'Amour KA, Agulnick AD, Eliazer S, Kelly OG, Kroon E, Baetge EE. Efficient differentiation of human embryonic stem cells to definitive endoderm.. Nat Biotechnol. 2005; 23(12):1534-41. (Methodology). 참조 보기
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Lai E, Prezioso VR, Tao WF, Chen WS, Darnell JE Jr. Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head. Genes Dev. 1991; 5(3):416-427. (Biology). 참조 보기
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Lin W, Metzakopian E, Mavromatakis YE, et al. Foxa1 and Foxa2 function both upstream of and cooperatively with Lmx1a and Lmx1b in a feedforward loop promoting mesodiencephalic dopaminergic neuron development.. Dev Biol. 2009; 333(2):386-396. (Biology). 참조 보기
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Monaghan AP, Kaestner KH, Grau E, Schütz G. Postimplantation expression patterns indicate a role for the mouse forkhead/HNF-3 alpha, beta and gamma genes in determination of the definitive endoderm, chordamesoderm and neuroectoderm.. Development. 1993; 119(3):567-578. (Biology). 참조 보기
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Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998; 282:1145-1147. (Methodology). 참조 보기
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