PE Mouse Anti-Human CD369 (Clec7A)

BD Pharmingen™ PE Mouse Anti-Human CD369 (Clec7A)

Name: PE Mouse Anti-Human CD369 (Clec7A)
Brand: BD Pharmingen™
SKU: 564471

Clone 15E2

(RUO)

Flow cytometric analysis of human CD369 (Clec7A) expression on peripheral blood leucocytes. Whole blood was stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 553457) or PE Mouse Anti-Human CD369 (Clec7A )antibody (Cat. No. 564471). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Contour plots showing the correlated expression patterns of CD369 (or Ig Isotype control staining) versus side light-scatter (SSC-A) characteristics were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Spectrum Viewer

Protocol Library

Scientific Resources

제품 세부 정보

Brand: BD Pharmingen™

Product Name: Hu CD369 (Clec7A) PE 15E2 100Tst

Alternative Name: CLEC7A; CLC7A; BGR; CLECSF12; CANDF4;Dectin-1

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: Human Dectin-1 Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Workshop Number: X 10-79

Entrez Gene ID: 64581

RRID: AB_2738819

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

제품 고시

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

관련 제품

Stain Buffer (FBS) RUO

사이즈 500 mL 카탈로그 No 554656

Stain Buffer (BSA) RUO

사이즈 500 mL 카탈로그 No 554657

Lysing Buffer RUO

사이즈 100 mL 카탈로그 No 555899

Lysing Solution 10X Concentrate IVD

사이즈 100 mL 카탈로그 No 349202

PE Mouse IgG2a, κ Isotype Control RUO

사이즈 0.1 mg 카탈로그 No 553457

564471 Rev. 2

항체 세부 정보

15E2

The 15E2 monoclonal antibody specifically binds to CD369 which is also known as CLEC7A (C-type lectin domain family 7 member A), Dectin-1 (Dendritic cell-associated C-type lectin 1), or BGR (Beta-glucan receptor). CD369 is a type II transmembrane C-type lectin that contains a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and an ITAM containing cytoplasmic tail. This protein is highly expressed on dendritic cells. It is also expressed on B cells, monocytes, eosinophils, mast cells, macrophages, endothelial cells, and is lowly expressed on some subsets of T cells. CD369 binds to beta-glucan polymers and functions as a pattern recognition receptor in innate immune response to fungal and bacterial pathogens. The 15E2 antibody can reportedly stimulate dendritic cells to costimulate immune responses.

564471 Rev. 2

형광 세부 정보

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

564471 Rev.2

인용 및 참고 문헌

개발 참고 자료 (5)

Hermanz-Falcon P, Arce I, Roda-Navarro P, Fernandez-Ruiz E. Cloning of human DECTIN-1, a novel C-type lectin-like receptor gene expressed on dendritic cells. Immunogenetics. 2001; 53(4):288-295. (Biology). 참조 보기
Ni L, Gayet I, Zurawski S, et al. Concomitant activation and antigen uptake via human dectin-1 results in potent antigen-specific CD8+ T cell responses. J Immunol. 2010; 185(6):3504-3513. (Immunogen: ELISA, Flow cytometry, Fluorescence microscopy, Functional assay, Immunofluorescence, Stimulation). 참조 보기
Sobanov Y, Bernreiter A, Derdak S, et al. A novel cluster of lectin-like receptor genes expressed in monocytic, dendritic and endothelial cells maps close to the NK receptor genes in the human NK gene complex. Eur J Immunol. 2001; 31(12):3493-3503. (Biology). 참조 보기
Willment JA, Gordon S, Brown GD. Characterization of the human beta -glucan receptor and its alternatively spliced isoforms. J Biol Chem. 2001; 276(47):43818-43823. (Biology). 참조 보기
Yokota K, Takashima A, Bergstresser PR, Ariizumi K. Identification of a human homologue of the dendritic cell-associated C-type lectin-1, dectin-1. Gene. 2001; 272(1-2):51-60. (Biology). 참조 보기

모두 보기 (5)

564471 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.