PE Mouse Anti-Human CD164

BD Pharmingen™ PE Mouse Anti-Human CD164

Name: PE Mouse Anti-Human CD164
Brand: BD Pharmingen™
SKU: 551298

Clone N6B6

(RUO)

Profile of peripheral blood monocytes analyzed by flow cytometry

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Scientific Resources

제품 세부 정보

Brand: BD Pharmingen™

Product Name: Hu CD164 PE N6B6 100Tst

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2a, κ

Immunogen: Human Pre-B Cell Line

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_394138

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

제품 고시

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

관련 제품

PE Mouse IgG2a, κ Isotype Control RUO

사이즈 100 Tests 카탈로그 No 555574

551298 Rev. 1

항체 세부 정보

N6B6

The N6B6 monoclonal antibody specifically recognizes CD164. CD164 is a 80-90 kDa, mucin-like molecule present on CD34+ cells during the early stages of B cell, erythroid cell and myelomonocytic cell development. It is also expressed on epithelial cells, peripheral blood monocytes and weakly on lymphocytes. Mucin-like molecules are a new family of glycoproteins present in tissues of the hematopoietic system. They are highly glycosylated polypeptides, containing predominantly O-linked carbohydrate side chains. CD164 may reportedly play a role in hematopoiesis by facilitating the adhesion of CD34+ cells to bone marrow stroma.

551298 Rev. 1

형광 세부 정보

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

551298 Rev.1

인용 및 참고 문헌

개발 참고 자료 (2)

Watt SM, Bühring HJ, Rappold I. CD164, a novel sialomucin on CD34(+) and erythroid subsets, is located on human chromosome 6q21. Blood. 1998; 92(3):849-866. (Biology). 참조 보기
Zannettino AC, Bühring HJ, Niutta S, Watt SM, Benton MA, Simmons PJ. The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood. 1998; 92(8):2613-2628. (Biology). 참조 보기

551298 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.