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Flow cytometric analysis of CD125 expression on human peripheral blood eosinophils. Human eosinophils were stained with either PE Mouse Anti-Human CD125 (Cat. No. 555902; solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was carried out on BD FACScan™ system.
BD Pharmingen™ PE Mouse Anti-Human CD125
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준비 및 보관
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The A14 monoclonal antibody specifically recognizes the α chain of the Interleukin-5 Receptor (IL-5Rα), which is also known as CD125. IL-5Rα is a 55-60 kDa type I transmembrane glycoprotein that is expressed on B1 B cells, eosinophils, basophils and mast cells. The IL-5Rα chain associates with CD131 (Common β-chain) which serves as the signaling common β-chain subunit of the IL-5R complex as well as for the Interleukin-3 (IL-3R) and GM-CSF receptor (GM-CSFR) complexes. IL-5 promotes the growth and differentiation of eosinophil precursors and activates mature eosinophils. Clone A14 was developed using IL-5Rα-transfected cells as immunogen. This antibody binding can be blocked by IL-5.
개발 참고 자료 (2)
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Blom M, Tool AT, Kok PT, Koenderman L, Roos D, Verhoeven AJ. Granulocyte-macrophage colony-stimulating factor, interleukin-3 (IL-3), and IL-5 greatly enhance the interaction of human eosinophils with opsonized particles by changing the affinity of complement receptor type 3. Blood. 1994; 83(10):2978-2984. (Biology). 참조 보기
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Ingley E, Young IG. Characterization of a receptor for interleukin-5 on human eosinophils and the myeloid leukemia line HL-60. Blood. 1991; 78(2):339-344. (Biology). 참조 보기
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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