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Flow cytometric analysis of CD122 expression on human peripheral blood lymphocytes. Whole blood was stained with either PE Mouse Anti-Human CD122 (Cat. No. 554525; solid line histogram) or PE Mouse IgG1 κ Isotype Control (Cat. No. 554680; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the forward and light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
BD Pharmingen™ PE Mouse Anti-Human CD122
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권장 분석 절차
This antibody has also been reported to be useful for immunoprecipitation experiments. It has been reported to precipitate from YTS cell lysates a 90,000 kD complex (IL-2 + p75). This antibody has also been reported not to block IL-2 binding to the β chain of the IL-2 receptor. Please note that this application is not routinely tested at BD Biosciences.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The Mik-β3 monoclonal antibody specifically binds to CD122. CD122 is also known as IL-2/15Rβ because it is the shared p75 subunit (IL-12Rβ/IL-15Rβ) of heteromeric receptors for IL-2 and IL-15. CD122 is a 70-75 kD type I transmembrane glycoprotein expressed on thymocytes, T cells, B cells, NK cells, monocytes, hematopoietic progenitor cells, fetal liver cells and stromal cells. The CD122 plays roles in the activation, differentiation and proliferation of various cell types including T cells, B cells and NK cells.
개발 참고 자료 (3)
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Ishikawa T, Uchiyama T, Kamio M, Onishi R, Kodaka T, Okuma M. IL-4 down-regulates IL-2 receptor p75 by accelerating its endocytosis. Int Immunol. 1991; 3(6):517-525. (Biology). 참조 보기
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Tsudo M, Kitamura F, Miyasaka M. Characterization of the interleukin 2 receptor beta chain using three distinct monoclonal antibodies. Proc Natl Acad Sci U S A. 1989; 86(6):1982-1986. (Biology). 참조 보기
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