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- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Two color flow cytometric analysis of FoxP1 expression in mouse thymocytes. Mouse thymocytes were stained with PerCP-Cy™5.5 Hamster Anti-Mouse CD3e antibody (Cat No. 561108)) and fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with either PE Mouse IgG2a, κ Isotype Control (Cat No. 554648, Left Panel) or PE Mouse Anti-FoxP1 antibody (Cat No. 564216; Right Panel). The flow cytometric dot plots show the correlated expression patterns of CD3 versus FoxP1 (or IgG2a Isotype Control staining) for gated events with the forward and side light-scatter characteristics of intact mouse thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
BD Pharmingen™ PE Mouse Anti-FoxP1
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준비 및 보관
권장 분석 절차
PE Mouse anti-FoxP1 antibody stained normal human peripheral blood B cells and T cells with a low signal-to-noise ratio (S/N ¡ 2) upon flow cytometric analyses. It is therefore not recommended for staining normal peripheral blood leucocytes for flow cytometric analysis.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The JC12 monoclonal antibody specifically binds to Forkhead box protein P1 (FoxP1). FoxP1 belongs to subfamily P of the forkhead box (FOX) winged-helix transcription factor family. It plays a variety of roles such as regulating the formation of lymphoid, lung, brain and heart tissues. FoxP1 is critical in regulating early B cell development and is also required for maintenance of naïve T cell quiescence, and monocyte differentiation and macrophage function. Aberrant expression of FOXP1 has been linked with mucosa-associated lymphoid tissue lymphoma and diffuse-large B cell lymphoma.
개발 참고 자료 (10)
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Banham AH, Beasley N, Campo E, et al. The FOXP1 winged helix transcription factor is a novel candidate tumor suppressor gene on chromosome 3p. Cancer Res. 2001; 61(24):8820-8829. (Immunogen: Immunohistochemistry, Western blot). 참조 보기
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Brown PJ, Ashe SL, Leich E, et al. Potentially oncogenic B-cell activation-induced smaller isoforms of FOXP1 are highly expressed in the activated B cell-like subtype of DLBCL. Blood. 2008; 111(5):2816-2824. (Clone-specific: Immunohistochemistry, Western blot). 참조 보기
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Feng X, Wang H, Takata H, Day TJ, Willen J, Hu H. Transcription factor Foxp1 exerts essential cell-intrinsic regulation of the quiescence of naive T cells. Nat Immunol. 2011; 12(6):544-550. (Biology). 참조 보기
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Hu H, Wang B, Borde M, et al. Foxp1 is an essential transcriptional regulator of B cell development. Nat Immunol. 2006; 7(8):819-826. (Biology). 참조 보기
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Katoh M, Igarashi M, Fukuda H, Nakagama H, Katoh M. Cancer genetics and genomics of human FOX family genes. Cancer Lett. 2013; 328(2):198-206. (Biology). 참조 보기
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Sagardoy A, Martinez-Ferrandis JI, Roa S, et al. Downregulation of FOXP1 is required during germinal center B-cell function. Blood. 2013; 121(21):4311-4320. (Biology). 참조 보기
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Shi C, Sakuma M, Mooroka T, et al. Down-regulation of the forkhead transcription factor Foxp1 is required for monocyte differentiation and macrophage function. Blood. 2008; 112(12):4699-4711. (Biology). 참조 보기
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Streubel B, Vinatzer U, Lamprecht A, Raderer M, Chott A. T(3;14)(p14.1;q32) involving IGH and FOXP1 is a novel recurrent chromosomal aberration in MALT lymphoma. Leukemia. 2005; 19(4):652-658. (Biology). 참조 보기
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Wang B, Lin D, Li C, Tucker P. Multiple domains define the expression and regulatory properties of Foxp1 forkhead transcriptional repressor. J Biol Chem. 2003; 278(27):24259-24268. (Biology). 참조 보기
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Wlodarska I, Veyt E, De Paepe P, et al. FOXP1, a gene highly expressed in a subset of diffuse large B-cell lymphoma, is recurrently targeted by genomic aberrations. Leukemia. 2005; 19(8):1299-1305. (Clone-specific: Immunohistochemistry). 참조 보기
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