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Two color flow cytometric analysis of CD272 expression on mouse bone marrow cells and splenocytes. Mouse bone marrow cells (Left Panel) were stained with PE Hamster Anti-Mouse CD272 (Cat. No. 563774) and APC-Cy™7 Rat Anti-Mouse CD45R/B220 (Cat. No. 552094). Splenic leucocytes (Right Panel) were stained with PE Hamster Anti-Mouse CD272 and BD Horizon™ V500 Syrian Hamster Anti-Mouse CD3e (Cat. No. 560771) antibodies. The two-color flow cytometric dot plot show the correlated expression patterns of either CD45R/B220 or CD3e versus CD272 for gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
BD Pharmingen™ PE Hamster Anti-Mouse CD272
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제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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관련 제품
The HMBT-6B2 monoclonal antibody specifically binds to CD272 which is also known as B- and T-lymphocyte attenuator (BTLA). CD272 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. CD272 is variably expressed on developing and mature T and B lymphocytes, NKT cells, NK cells, macrophages and dendritic cells. CD272 expression is upregulated by activated T cells including Th1, Th2, and T follicular helper cells and by anergic T cells. CD272 is structurally similar to CD152/CTLA-4 and CD279/PD-1 and functions as a coinhibitory receptor for B and T cell responses. Herpesvirus entry mediator (HVEM), also known as CD270 and LIGHT-R, has been identified as a ligand for CD272. The crosslinking of CD272 by HVEM inhibits T-cell proliferation and cytokine production.
개발 참고 자료 (7)
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Han P, Goularte OD, Rufner K, Wilkinson B, Kaye J. An inhibitory Ig superfamily protein expressed by lymphocytes and APCs is also an early marker of thymocyte positive selection. J Immunol. 2004; 172(10):5931-5939. (Biology). 참조 보기
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Hurchla MA, Sedy JR, Gavrieli M, Drake CG, Murphy TL, Murphy KM. B and T lymphocyte attenuator exhibits structural and expression and is highly induced in anergic CD4+T cells. J Immunol. 2005; 174(6):3377-3385. (Biology). 참조 보기
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Ishida W, Fukuda K, Kajisako M, et al. B and T lymphocyte attenuator regulates the development of antigen-induced experimental conjunctivitis. Graefes Arch Clin Exp Ophthalmol. 2012; 250(2):289-295. (Immunogen: In vivo exacerbation). 참조 보기
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Nurieva RI, Chung Y, Martinez GJ, et al. Bcl6 mediates the development of T follicular helper cells. Science. 2009; 325(5943):1001-1005. (Biology). 참조 보기
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Sedy JR, Gavrieli M, Potter KG, et al. B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator. Nat Immunol. 2005; 6(1):90-98. (Biology). 참조 보기
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Watanabe N, Gavrieli M, Sedy JR, et al. BTLA is a lymphocyte inhibitory receptor with similarities to CTLA-4 and PD-1. Nat Immunol. 2003; 4(7):670-679. (Biology). 참조 보기
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Watanabe N, Nakajima H. Coinhibitory molecules in autoimmune diseases. Clin Dev Immunol. 2012; 2012:1-7. (Biology). 참조 보기
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