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Two-color analysis of the expression of Vβ 14 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated 14-2 and PE-conjugated 145-2C11 (anti-CD3e, Cat. No. 553063/553064) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
BD Pharmingen™ FITC Rat Anti-Mouse Vβ 14 T-Cell Receptor
규제 상태 범례
Becton, Dickinson and Company의 명시적인 서면 승인 없이는 사용 하실 수 없습니다.
준비 및 보관
권장 분석 절차
For flow cytometry of cell suspensions from peripheral lymphoid tissue, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
The 14-2 antibody reacts with the Vβ 14 T-Cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) halpotypes of the Tcrb gene complex. Vβ 14 TCR-expressing T lymphocytes are completely eliminated in mice expressing I-E and the superantigens encoded by Mtv-2 endogenous provirus and/or MMTV-C3H, MMTV-GR, or MMTV-D2.GD exogenous virus. Recognition of these determinants by Vβ 14 TCR-expressing T cells is dependent upon presentation by I-E. Plate bound 14-2 antibody activates Vβ 14 TCR-bearing T cells.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
개발 참고 자료 (7)
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Acha-Orbea H, Shakhov AN, Scarpellino L, et al. Clonal deletion of V beta 14-bearing T cells in mice transgenic for mammary tumour virus. Nature. 1991; 350(6315):207-211. (Biology). 참조 보기
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Choi Y, Kappler JW, Marrack P. A superantigen encoded in the open reading frame of the 3' long terminal repeat of mouse mammary tumour virus. Nature. 1991; 350(6315):203-207. (Biology). 참조 보기
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Golovkina TV, Chervonsky A, Dudley JP, Ross SR. Transgenic mouse mammary tumor virus superantigen expression prevents viral infection. Cell. 1992; 69(4):637-645. (Clone-specific). 참조 보기
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Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology). 참조 보기
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Liao NS, Maltzman J, Raulet DH. Positive selection determines T cell receptor V beta 14 gene usage by CD8+ T cells. J Exp Med. 1989; 170(1):135-143. (Immunogen). 참조 보기
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Marrack P, Kushnir E, Kappler J. A maternally inherited superantigen encoded by a mammary tumour virus. Nature. 1991; 349(6309):524-526. (Biology). 참조 보기
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Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). 참조 보기
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