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Flow cytometric analysis for T- and B- Cell Activation Antigen in activated mouse spleen cells. Concanavalin A-stimulated (3 days) mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either FITC Rat IgM, κ Isotype Control (Cat No. 553942; dashed line histogram) or with the FITC Rat Anti-Mouse T- and B- Cell Activation Antigen antibody (Cat No. 553666/562080; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
BD Pharmingen™ FITC Rat Anti-Mouse T- and B-Cell Activation Antigen
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제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
관련 제품
The GL7 antibody specifically recognizes the T- and B- Cell Activation Antigen which is also known as, the GL7 antigen. The GL7 antigen is a 35-kDa cell-surface protein that is expressed on T and B lymphocytes activated in vitro, on bone marrow Pre-B-II cells, germinal-center B cells, and the subpopulation of thymocytes that coexpress high CD3e levels. The GL7 antibody recognizes an epitope containing nonsulfated α2-6-sialyl-LacNAc. There is strain variability with respect to GL7 antigen distribution on thymocytes and Con A-activated spleen cells. GL7 antigen expression is found to be higher on BALB/c mouse leucocytes than on C57BL/6 mouse counterparts. The GL7 antibody reportedly may crossreact with epitopes on molecules expressed by certain rat and human leucocyte subsets.
개발 참고 자료 (5)
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Han S, Dillon SR, Zheng B, Shimoda M, Schlissel MS, Kelsoe G. V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science. 1997; 278(5336):301-305. (Biology). 참조 보기
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Han S, Zheng B, Schatz DG, Spanopoulou E, Kelsoe G. Neoteny in lymphocytes: Rag1 and Rag2 expression in germinal center B cells. Science. 1996; 274(5295):2094-2097. (Biology). 참조 보기
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Han S, Zheng B, Takahashi Y, Kelsoe G. Distinctive characteristics of germinal center B cells. Semin Immunol. 1997; 9(4):255-260. (Biology). 참조 보기
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Hathcock KS, Pucillo CE, Laszlo G, Lai L, Hodes RJ. Analysis of thymic subpopulations expressing the activation antigen GL7. Expression, genetics, and function. J Immunol. 1995; 155(10):4575-4581. (Biology). 참조 보기
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Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993; 150(12):5252-5262. (Immunogen). 참조 보기
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