FITC Mouse Anti-Mouse IgM[b]

BD Pharmingen™ FITC Mouse Anti-Mouse IgM[b]

Name: FITC Mouse Anti-Mouse IgM[b]
Brand: BD Pharmingen™
SKU: 553520

Clone AF6-78

(RUO)

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Scientific Resources

제품 세부 정보

Brand: BD Pharmingen™

Product Name: Ms IgMb FITC AF6-78 500ug

Alternative Name: Igh-6b

Reactivity: Mouse (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: C57BL/10 mouse splenocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_394901

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

권장 분석 절차

FITC-conjugated AF6-78 mAB may be used as a primary or secondary reagent in immunofluorescent staining.

제품 고시

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

관련 제품

FITC Mouse IgG1, κ Isotype Control RUO

사이즈 0.25 mg 카탈로그 No 550616

553520 Rev. 8

항체 세부 정보

AF6-78

The AF6-78 antibody reacts specifically with mouse IgM of Igh-C[b] and related haplotypes (e.g., C57BL/6, C57BL/10, SJL, AKR, NZB). It does not react with IgM of Igh-C[a] or related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2, PL, RIII). Cross-reaction with IgM of Igh-C[e] haplotype (e.g., A/J) has been observed. AF6-78 antibody does not react with free µ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain. It has not been shown to stimulate B-cell proliferation.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553520 Rev. 8

형광 세부 정보

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

형광: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

553520 Rev.8

인용 및 참고 문헌

개발 참고 자료 (2)

Stall AM, Loken MR. Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies. J Immunol. 1984; 132(2):787-795. (Immunogen). 참조 보기
Stall AM. Mouse immunoglobulin allotypes. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology. Blackwell Science Publishers; 1996:27.1-27.16.

553520 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.