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Profile of peripheral blood monocytes analyzed by flow cytometry
BD Pharmingen™ FITC Mouse Anti-Human CD329 (Siglec-9)
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제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The E10-286 monoclonal antibody specifically binds to CD329. CD329 is also known as Sialic acid-binding Ig-like lectin 9 (Siglec-9), formerly known as Siglec-Y, which is expressed on peripheral blood monocytes, granulocytes and NK cells. Siglecs (sialic acid/immunolgobulin/lectin) are a family of I-type lectins binding to sialic acids on the cell surface. They are a family of carbohydrate-binding proteins within the immunoglobulin superfamily. Siglecs are all integral membrane proteins with extracellular domains consisting of unusual N-termial V-set Ig domains, followed by variable numbers of C2-set Ig domains.
개발 참고 자료 (2)
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Powell LD, Varki A. I-type lectins. J Biol Chem. 1995; 270(24):14243-14246. (Biology). 참조 보기
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Varki A, Chrispeels MJ. Varki, A., Esko, J. Cummings, R. Ajit Varki .. et al. ; with contributions from Maarten Chrispeels .. et al., ed. Essentials of glycobiology. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press; 1999:101.
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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