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BD OptiBuild™ BV786 Rat Anti-Mouse CD335 (NKp46)

Name: BV786 Rat Anti-Mouse CD335 (NKp46)
Brand: BV786 Rat Anti-Mouse CD335 (NKp46)
SKU: 741029

Clone 29A1.4 (RUO)

Multiparameter flow cytometric analysis using BD OptiBuild™ BV786 Rat Anti-Mouse CD335 (NKp46) antibody (Cat. No. 741029) on live mouse splenocytes. Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

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Scientific Resources

제품 세부 정보

Brand: BD OptiBuild™

Alternative Name: Ncr1; NK-p46; NKp46; mNKp46; MAR1; mAR-1; mouse activating receptor 1; Ly94

Reactivity: Mouse (Tested in Development)

Isotype: Rat IgG2a, κ

Immunogen: Mouse NKP46 Recombinant Protein

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

RRID: AB_2740647

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

준비 및 보관

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.

권장 분석 절차

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

제품 고시

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
Cy is a trademark of Amersham Biosciences Limited.

관련 제품

Stain Buffer (FBS) RUO

사이즈 500 ML 카탈로그 No 554656

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

사이즈 0.1 mg 카탈로그 No 553141

Stain Buffer (BSA) RUO

사이즈 500 ML 카탈로그 No 554657

Brilliant Stain Buffer RUO

사이즈 100 Tests 카탈로그 No 563794

Lysing Buffer RUO

사이즈 100 ML 카탈로그 No 555899

BV786 Rat IgG2a, κ Isotype Control RUO

사이즈 50 µg 카탈로그 No 563335

세부 정보보기

741029 Rev. 2

항체 세부 정보

29A1.4

The monoclonal antibody 29A1.4 specifically binds to mouse CD335, also known as NKp46. NKp46 is a 46 kDa type I transmembrane glycoprotein that is a member of the natural cytotoxicity receptor (NCR) family and immunoglobulin superfamily. NKp46 is encoded by the Ncr1 gene located on chromosome 7. NKp46 functions as a cytotoxicity triggering receptor and is selectively expressed by immature and mature NK cells in all mouse strains tested. NKp46 is detected on a minute fraction of NK-like T cells (less than 2% of NKp46+ express CD3e) but not on CD1d-restricted NKT cells from C57BL/6 mice. When immobilized on tissue culture plates, the 29A1.4 antibody reportedly stimulates NK cells to produce interferon-gamma and to release their cytoplasmic granule contents. Although the ligands for the NKp46 receptor have not been fully characterized, recent evidence indicates that this receptor plays an important role in the NK cell-mediated recognition and killing of some virus-infected cells and tumor cells. The immunogen used to generate the 29A1.4 clone was mouse NKp46-Fc recombinant protein.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

741029 Rev. 2

형광 세부 정보

BV786

The BD Horizon Brilliant Violet™ 786 (BV786) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an Ex Max of 407-nm and an acceptor dye with an Em Max at 786-nm. BV786, driven by BD innovation, is designed to be excited by the violet laser and detected using a filter, centered near 785 nm (e.g. 780/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and filters) are appropriate for this dye.

형광: BV786

Excitation Max: 407 nm

Emission Max: 786 nm

741029 Rev.2

인용 및 참고 문헌

View product citations for antibody "741029" on CiteAb

개발 참고 자료 (4)

Biassoni R, Pessino A, Bottino C, Pende D, Moretta L, Moretta A. The murine homologue of the human NKp46, a triggering receptor involved in the induction of natural cytotoxicity. Eur J Immunol. 1999; 29(3):1014-1020. (Biology). 참조 보기
Gazit R, Gruda R, Elboim M, et al. Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1. Nat Immunol. 2006; 7(5):517-523. (Biology). 참조 보기
Joncker NT, Fernandez NC, Treiner E, Vivier E, Raulet DH. NK cell responsiveness is tuned commensurate with the number of inhibitory receptors for self-MHC class I: the rheostat model. J Immunol. 2009; 182(8):4572-4580. (Clone-specific: Flow cytometry). 참조 보기
Walzer T, Blery M, Chaix J, et al. Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46. Proc Natl Acad Sci U S A. 2007; 104(9):3384-3389. (Immunogen: Activation, Flow cytometry). 참조 보기

모두 보기 (4)

741029 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.