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      BV510 Rat Anti-Mouse IL-17A
      BV510 Rat Anti-Mouse IL-17A
      Flow cytometric analysis of IL-17A-producing mouse EL-4 cells. Cells from the mouse EL4 (T lymphoma, ATCC TIB-39) cell line were stimulated (5 hr) with Phorbol 12-Myristate 13-Acetate (PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139) and Ionomycin (1000 ng/ml final concentration; Sigma, Cat. No. I-0634) in the presence of BD GolgiStop™ Protein Transport Inhibitor (containing Monensin) (Cat. No. 554724). The cells were washed with BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656), preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142), and fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722).        The cells were then washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with BD Horizon™ BV510 Rat IgG1 κ Isotype Control (Cat. No. 563039; Left Panel) or BD Horizon BV510 Rat Anti-Mouse IL-17A antibody (Cat. No. 564168; Right Panel) using BD Biosciences' Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color contour plots showing correlated expression patterns of IL-17A (or Ig Isotype control staining) versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      BV510 Rat Anti-Mouse IL-17A
      Flow cytometric analysis of IL-17A-producing mouse EL-4 cells. Cells from the mouse EL4 (T lymphoma, ATCC TIB-39) cell line were stimulated (5 hr) with Phorbol 12-Myristate 13-Acetate (PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139) and Ionomycin (1000 ng/ml final concentration; Sigma, Cat. No. I-0634) in the presence of BD GolgiStop™ Protein Transport Inhibitor (containing Monensin) (Cat. No. 554724). The cells were washed with BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656), preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142), and fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722).        The cells were then washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with BD Horizon™ BV510 Rat IgG1 κ Isotype Control (Cat. No. 563039; Left Panel) or BD Horizon BV510 Rat Anti-Mouse IL-17A antibody (Cat. No. 564168; Right Panel) using BD Biosciences' Protocol for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Two-color contour plots showing correlated expression patterns of IL-17A (or Ig Isotype control staining) versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      제품 세부 정보
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      BD Horizon™
      Ms IL-17A BV510 TC11-18H10 50ug
      IL-17; Il17; IL-17A; Il17a; CTLA-8; Ctla8; Interleukin-17A; interleukin 17A
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG1, κ
      Recombinant Mouse IL-17A Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      AB_2738639
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      준비 및 보관

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.
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      제품 고시

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Brilliant Violet™ 510 is a trademark of Sirigen.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      관련 제품

      Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
      사이즈 0.1 mg 카탈로그 No 553141
      sampleImage/
      Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
      사이즈 0.5 mg 카탈로그 No 553142
      sampleImage/
      Stain Buffer (FBS) RUO
      사이즈 500 mL 카탈로그 No 554656
      sampleImage/
      Fixation and Permeabilization Solution RUO
      사이즈 125 mL 카탈로그 No 554722
      sampleImage/
      Perm/Wash Buffer RUO
      사이즈 100 mL 카탈로그 No 554723
      sampleImage/
      Protein Transport Inhibitor (Containing Monensin) RUO
      사이즈 0.7 mL 카탈로그 No 554724
      sampleImage/
      세부 정보보기
      564168 Rev. 1
      항체 세부 정보
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      TC11-18H10

      The TC11-18H10 monoclonal antibody specifically binds to recombinant and natural mouse IL-17A proteins. IL-17A, also known as CTLA-8, is a T cell-derived cytokine that promotes inflammatory responses.  Mouse IL-17A is a proinflammatory cytokine that can induce the release of IL-6 by mouse stromal cells. It has been shown to support the growth of hemopoietic progenitors in vitro; it can also stimulate granulopoiesis in vivo. The TC11-18H10 antibody has been reported to neutralize IL-17A activity.  Recent studies have shown that IL-17A is produced by a unique subset of Th17 cells that develop along a pathway distinct from the Th1- and Th2- cell differentiation pathways. The mouse IL-17A cDNA was isolated from a cDNA library generated from TCRαβ+CD4-CD8- thymocytes.

      The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

      564168 Rev. 1
      형광 세부 정보
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      BV510
      The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV510
      Violet 405 nm
      327 nm, 405 nm
      512 nm
      564168 Rev.1
      인용 및 참고 문헌
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      개발 참고 자료 (4)

      1. Coquet JM, Chakravarti S, Kyparissoudis K, et al. Diverse cytokine production by NKT cell subsets and identification of an IL-17-producing CD4-NK1.1- NKT cell population. Proc Natl Acad Sci U S A. 2008; 105(32):11287-11292. (Clone-specific: Flow cytometry). 참조 보기
      2. Dong C. Th17 cells: Current understanding of their generation and regulation. Eur J Immunol. 2009; 39(3):640-644. (Biology). 참조 보기
      3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry). 참조 보기
      4. Schwarzenberger P, La Russa V, Miller A, et al. IL-17 stimulates granulopoiesis in mice: use of an alternate, novel gene therapy-derived method for in vivo evaluation of cytokines. J Immunol. 1998; 161(11):6383-6389. (Biology). 참조 보기
      모두 보기 (4) 간단히 보기
      564168 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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