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권장 분석 절차
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
제품 고시
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The 6C3 monoclonal antibody specifically recognizes CD249 which is also known as, 6C3/BP-1, Enpep, or Ly-51. CD249 is a glycoprotein cell-surface differentiation antigen, which was originally identified on pre-B cell lymphomas (spontaneous and chemical- or retrovirus-transformed, in vitro and in vivo). CD249 is a homodimeric cell-surface glycoprotein with 140-kDa subunits that has been identified to possess aminopeptidase A (APA) activity. The same antigen is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis, on thymic dendritic cells and cortical epithelial cells, and on a wide variety of mouse and rat tissues known to possess APA activity. Subsets of normal bone marrow pre-B and B lymphocytes express low levels of CD249 which is rapidly up-regulated on the pre-B cells in the presence of IL-7. A role for the CD249 molecule in the IL-7-driven proliferation of B cell precursors has been postulated. However, B-cell abnormalities were not detected in CD249/Ly-51-deficient mice. Mature B lymphocytes, thymocytes, peripheral T lymphocytes, erythroid cells, and myeloid cells (with the exception of thymic dendritic cells) do not express CD249. The 6C3 antibody can be used to identify cortical epithelium in frozen sections of thymuses from normal, SCID, and TCR-transgenic mice. It is possible that the low level of CD249 antigen detected, by flow cytometry, on some thymocytes may be passively adsorbed from adjacent epithelial cells during preparation of the cell suspensions.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously.
개발 참고 자료 (19)
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