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- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Multicolor flow cytometric analysis of CD286 (TLR6) expression on mouse macrophages. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse F4/80 (Cat. No. 565410) and Alexa Fluor® 647 Rat Anti-Mouse CD11b (Cat. No. 557686) antibodies, and either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BV421 Mouse Anti-Mouse CD286 (TLR6) antibody (Cat. No. 566005; solid line histogram). The fluorescence histogram showing CD286 (TLR6) expression (or Ig Isotype control staining) was derived from CD11b-hi F4/80-hi events with the forward and side light-scatter characteristics of viable bone marrow myeloid cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis of CD286 (TLR6) expression on mouse RAW 264.7 cells. Cells from the RAW 264.7 (Macrophage ATCC TIB-71) cell line were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either BD Horizon BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BV421 Mouse Anti-Mouse CD286 (TLR6) antibody (Cat. No. 566005; solid line histogram). The fluorescence histogram showing CD286 (TLR6) expression (or Ig Isotype control staining), was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
BD Horizon™ BV421 Mouse Anti-Mouse CD286 (TLR6)
BD Horizon™ BV421 Mouse Anti-Mouse CD286 (TLR6)
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권장 분석 절차
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- An isotype control should be used at the same concentration as the antibody of interest.
관련 제품
The C1N2 monoclonal antibody specifically binds to the Toll-like receptor 6 (TLR6), which is also known as CD286. CD286 belongs to the mammalian toll-like family of pattern recognition receptors (TLR) that participate in inducing innate immune responses to microbial pathogens. CD286 is a type I transmembrane glycoprotein comprised of an extracellular domain with a large number of leucinerich repeats (LRRs) and a cytoplasmic region with a signaling Toll/Interleukin-1 Receptor (TIR) domain. TLR6 is differentially expressed on the surface and intracellular compartments, including endolysosomes, of pre-B cells as well as immature or mature B cells. It is likewise expressed by monocytes, macrophages, or plasmacytoid, CD4+, or CD8+ dendritic cells. In association with TLR2 (CD282), CD286 recognizes microbial diacylated lipopeptides, lipoteichoic acid, and zymosan. The CD282/CD286 (TLR2/TLR60 heterodimer then transduces signaling responses leading to the production of proinflammatory cytokines by responding cells.
개발 참고 자료 (5)
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Kang JY, Lee JO. Structural biology of the Toll-like receptor family.. Annu Rev Biochem. 2011; 80:917-41. (Biology). 참조 보기
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Marinho FA, de Paula RR, Mendes AC, et al. Toll-like receptor 6 senses Mycobacterium avium and is required for efficient control of mycobacterial infection.. Eur J Immunol. 2013; 43(9):2373-85. (Biology). 참조 보기
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Motoi Y, Shibata T, Takahashi K, et al. Lipopeptides are signaled by Toll-like receptor 1, 2 and 6 in endolysosomes.. Int Immunol. 2014; 26(10):563-73. (Immunogen: Flow cytometry, Immunoprecipitation). 참조 보기
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Takeuchi O, Kawai T, Sanjo H, et al. TLR6: A novel member of an expanding toll-like receptor family. Gene. 1999; 231(1-2):59-65. (Biology). 참조 보기
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de Almeida LA, Macedo GC, Marinho FA, et al. Toll-like receptor 6 plays an important role in host innate resistance to Brucella abortus infection in mice.. Infect Immun. 2013; 81(5):1654-62. (Biology). 참조 보기
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