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      BV421 Mouse Anti-Human TRA-1-85 Antigen
      BV421 Mouse Anti-Human TRA-1-85 Antigen
      Flow cytometric analysis of TRA-1-85 expression on human peripheral blood lymphocytes. Human whole blood was stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human TRA-1-85 Antigen antibody (Cat. No. 563302; solid line histogram). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).  The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      BV421 Mouse Anti-Human TRA-1-85 Antigen
      Flow cytometric analysis of TRA-1-85 expression on human peripheral blood lymphocytes. Human whole blood was stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human TRA-1-85 Antigen antibody (Cat. No. 563302; solid line histogram). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).  The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      제품 세부 정보
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      BD Horizon™
      Hu TRA-1-85 Ag BV421 TRA-1-85 50Tst
      OKa, CD147, EMMPRIN, Basigin
      Human (QC Testing)
      Mouse IgG1, κ
      Human Embryonal Carcinoma Cell Line
      Flow cytometry (Routinely Tested)
      5 µl
      AB_2738127
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      준비 및 보관

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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      제품 고시

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      6. Brilliant Violet™ 421 is a trademark of Sirigen.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      563302 Rev. 1
      항체 세부 정보
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      TRA-1-85

      The TRA-1-85 monoclonal antibody (mAb) specifically binds to the high frequency blood group antigen OKa. The OKa blood group antigen is a 35-69 kDa glycoprotein also known as CD147, EMMPRIN, M6, or Basigin. The OKa group is widely expressed on most human tissues including, but not limited to kidney, peripheral blood leukocytes, liver, pancreas, colon, skin, and brain. TRA-1-85 mAb is often used to discern human cells from non-human cells in multiple applications including animal transplantation studies. During development, the purified TRA-1-85 mAb was found to detect OKa blood group antigen by western blot analysis of cellular lysates, by immunofluorescent staining of fixed cells, and by flow cytometric analysis of live cells.

      The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

      563302 Rev. 1
      형광 세부 정보
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      563302 Rev.1
      인용 및 참고 문헌
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      개발 참고 자료 (5)

      1. Choi KD, Yu J, Smuga-Otto K, et al. Hematopoietic and endothelial differentiation of human induced pluripotent stem cells. Stem Cells. 2009; 27(3):559-567. (Clone-specific: Flow cytometry). 참조 보기
      2. Draper JS, Pigott C, Thomson JA, Andrews PW. Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat. 2002; 200:249-258. (Clone-specific: Flow cytometry). 참조 보기
      3. Olivier EN, Rybicki AC, Bouhassira EE. Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells. 2006; 24(8):1914-1922. (Biology). 참조 보기
      4. Spring FA, Holmes CH, Simpson KL, et.al. The Oka blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, basigin and neurothelin, an immunoglobulin superfamily molecule that is widely expressed in human cells and tissues. Eur J Immunol. 1997; 27(4):891-897. (Clone-specific: Flow cytometry, Immunohistochemistry, Western blot). 참조 보기
      5. Williams BP, Daniels GL, Pym B, et al. Biochemical and genetic analysis of the OKa blood group antigen. Immunogenetics. 1988; 27:322-329. (Clone-specific: Flow cytometry). 참조 보기
      모두 보기 (5) 간단히 보기
      563302 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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