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- BD® AbSeq Assay
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- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Two-color analysis of the expression of Vb 4 TCR on peripheral T lymphocytes. SJL lymph node cells were incubated simultaneously with biotinylated KT4 (Panel B), PE-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. no. 554057, both panels). Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
BD Pharmingen™ Biotin Rat Anti-Mouse Vβ4 T-Cell Receptor
규제 상태 범례
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준비 및 보관
권장 분석 절차
For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
관련 제품
The KT4 antibody specifically recognizes the Vβ4 T-cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ4 TCR-bearing cells are among the CD4+ autoreactive T cells which induce autoimmune thyroiditis after elimination of regulatory (CD5-bright) T-cell subsets. Plate-bound KT4 antibody activates Vβ4 TCR-bearing T cells.
개발 참고 자료 (3)
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Katz JD, Benoist C, Mathis D. T helper cell subsets in insulin-dependent diabetes. Science. 1995; 268(5214):1185-1188. (Biology). 참조 보기
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Sugihara S, Fujiwara H, Shearer GM. Autoimmune thyroiditis induced in mice depleted of particular T cell subsets. Characterization of thyroiditis-inducing T cell lines and clones derived from thyroid lesions. J Immunol. 1993; 150(2):683-694. (Biology). 참조 보기
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Tomonari K, Lovering E, Spencer S. Correlation between the V beta 4+ CD8+ T-cell population and the H-2d haplotype. Immunogenetics. 1990; 31(5-6):333-339. (Immunogen). 참조 보기
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