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Immunohistochemistry of H-2Kb positive cells. Frozen sections of mouse thymus were reacted with the anti-H2Kb antibody. Cells expressing the MHC class I molecule H-2Kb can be identified by the intense brown labeling of their cell membranes. Magnification 20X.
BD Pharmingen™ Biotin Mouse Anti-Mouse H-2K[b]
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준비 및 보관
권장 분석 절차
The AF6-88.5 clone reactive against mouse H-2Kb is recommended to test for immunohistorical staining of acetone-fixed frozen sections. Tissues tested were mouse (C57B1/6) spleen and thymus. The antibody stains the H-2Kb molecules found on the surface of all nucleated cells in strains of mice expressing this haplotype. For optimal immunohistochemical staining, the biotinylated antibody should be titrated (1:10 to 1:50 dilution) and developed with Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone AF6-88.5 is not recommended for formalin-fixed paraffin embedded sections.
제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.
- This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The AF6-88.5 antibody recognizes the H-2Kb MHC class I alloantigen. Reactivity with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v) has not been observed.
개발 참고 자료 (2)
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Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J Immunol Methods. 1982; 50(3):R85-R112. (Immunogen). 참조 보기
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Wall KA, Lorber MI, Loken MR, McClatchey S, Fitch FW. Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.. J Immunol. 1983; 131(3):1056-64. (Biology). 참조 보기
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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