Flow cytometric analysis of CD44 expression on human peripheral blood lymphocytes. Whole blood was stained with either Biotin Mouse Anti-Human CD44 (Cat. No. 555477, solid line histogram) or Biotin Mouse IgG2b ĸ Isotype Control (Cat. No. 555741, dashed line histogram), followed by PE-Streptavidin (Cat. No. 554061, both histograms). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Flow cytometric analysis of CD44 expression on human peripheral blood lymphocytes. Whole blood was stained with either Biotin Mouse Anti-Human CD44 (Cat. No. 555477, solid line histogram) or Biotin Mouse IgG2b ĸ Isotype Control (Cat. No. 555741, dashed line histogram), followed by PE-Streptavidin (Cat. No. 554061, both histograms). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
제품 세부 정보
BD Pharmingen™
Hu CD44 Biotin G44-26 100Tst
Phagocytic glycoprotein 1; Pgp-1; H-CAM; Hermes; ECMR III; HUTCH-1
Human (QC Testing)
Mouse IgG2b, κ
Flow cytometry (Routinely Tested)
20 µl
VI A092
960
AB_395869
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
준비 및 보관
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
Stain Buffer (BSA) RUO
사이즈
500 mL
카탈로그 No
554657
Stain Buffer (FBS) RUO
사이즈
500 mL
카탈로그 No
554656
Lysing Solution 10X Concentrate IVD
사이즈
100 mL
카탈로그 No
349202
Lysing Buffer RUO
사이즈
100 mL
카탈로그 No
555899
Biotin Mouse IgG2b, κ Isotype Control RUO
사이즈
100 Tests
카탈로그 No
555741
PE Streptavidin RUO
사이즈
0.5 mg
카탈로그 No
554061
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555477 Rev. 3
항체 세부 정보
G44-26
The G44-26 monoclonal antibody specifically binds to the 80-95 kDa glycosylated type I transmembrane protein, CD44, also known as phagocytic glycoprotein-1 (Pgp-1). CD44 is the receptor for hyaluronic acid. CD44 is expressed on leucocytes, erythrocytes, epithelial cells and weakly on platelets. CD44 is also called extracellular matrix receptor type III and has functional roles in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation. This antibody recognizes epitope 1 of CD44 antigen according to the HLDA workshop studies.
555477 Rev. 3
형광 세부 정보
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
555477 Rev.3
인용 및 참고 문헌
개발 참고 자료 (5)
-
Galandrini R, Galluzzo E, Albi N, Grossi CE, Velardi A. Hyaluronate is costimulatory for human T cell effector functions and binds to CD44 on activated T cells. J Immunol. 1994; 153(1):21-31. (Biology). 참조 보기
-
Günthert U. CD44: a multitude of isoforms with diverse functions. Curr Top Microbiol Immunol. 1993; 184:47-63. (Biology). 참조 보기
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Patel DD, Liao HX, Haynes BF. CD44 workshop panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:373-375.
-
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
-
Stamenkovic I, Amiot M, Pesando JM, Seed B. A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family. Cell. 1989; 56(6):1057-1062. (Biology). 참조 보기
555477 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
