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Reagents
- Flow Cytometry Reagents
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Western Blotting and Molecular Reagents
- Immunoassay Reagents
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Functional Assays
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Microscopy and Imaging Reagents
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ Accessory Kits
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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준비 및 보관
권장 분석 절차
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD Optibuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
When setting up compensation, it is recommended to compare spillover values obtained from cells and BD™ CompBeads to ensure that beads will provide sufficiently accurate spillover values.
For optimal results, it is recommended to perform two washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescent staining prior to acquisition on a flow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population.
제품 고시
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Blue 700 is covered by one or more of the following US patents: 8,455,613 and 8,575,303.
- Cy is a trademark of GE Healthcare.
- The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. Diagnostic uses require a separate license.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
관련 제품
The TM-β1 monoclonal antibody specifically recognizes the 90-100-kDa β chain shared by the IL-2 and IL-15 receptors (IL-2Rβ, CD122). In the periphery, CD122 is expressed on CD8+ T lymphocytes, NK cells, NK-T cells, dendritic epidermal T cells, subsets of intraepithelial lymphocytes, and macrophages. Small subsets of fetal and adult thymocytes constitutively express CD122. CD122+ cells in the bone marrow include committed NK-cell progenitors. IL-2Rβ expression is upregulated by IL-2. CD122 is a transmembrane glycoprotein of the hematopoietin receptor superfamily which can combine with CD132 (γc) alone or CD132 plus CD25 (IL-2Rα) to form intermediate or high-affinity IL-2 receptor complexes, respectively. The β chain of these complexes, CD122, is involved in signal transduction and immunoregulation. The TM-β1 antibody blocks high affinity binding of IL-2 or IL-15 to IL-2Rβ.
The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes. It is a polymer-based tandem dye developed exclusively by BD Biosciences. With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40-nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.
개발 참고 자료 (18)
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Alleva DG, Kaser SB, Monroy MA, Fenton MJ, Beller DI. IL-15 functions as a potent autocrine regulator of macrophage proinflammatory cytokine production: evidence for differential receptor subunit utilization associated with stimulation or inhibition. J Immunol. 1997; 159(6):2941-2951. (Clone-specific: Blocking). 참조 보기
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Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology). 참조 보기
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Cho BK, Wang C, Sugawa S, Eisen HN, Chen J. Functional differences between memory and naive CD8 T cells. Proc Natl Acad Sci U S A. 1999; 96(6):2976-2981. (Biology). 참조 보기
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Giri JG, Ahdieh M, Eisenman J, et al. Utilization of the beta and gamma chains of the IL-2 receptor by the novel cytokine IL-15. EMBO J. 1994; 13(12):2822-2830. (Biology). 참조 보기
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Guy-Grand D, Cuenod-Jabri B, Malassis-Seris M, Selz F, Vassalli P. Complexity of the mouse gut T cell immune system: identification of two distinct natural killer T cell intraepithelial lineages. Eur J Immunol. 1996; 26(9):2248-2256. (Clone-specific: Flow cytometry). 참조 보기
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Hanke T, Mitnacht R, Boyd R, Hunig T. Induction of interleukin 2 receptor beta chain expression by self-recognition in the thymus. J Exp Med. 1994; 180(5):1629-1636. (Clone-specific: Flow cytometry). 참조 보기
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Kondo M, Ohashi Y, Tada K, Nakamura M, Sugamura K. Expression of the mouse interleukin-2 receptor gamma chain in various cell populations of the thymus and spleen. Eur J Immunol. 1994; 24(9):2026-2030. (Biology). 참조 보기
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Ku CC, Murakami M, Sakamoto A, Kappler J, Marrack P. Control of homeostasis of CD8+ memory T cells by opposing cytokines. Science. 2000; 288(5466):675-678. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). 참조 보기
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Malek TR, Furse RK, Fleming ML, Fadell AJ, He YW. Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits. J Interferon Cytokine Res. 1995; 15(5):447-454. (Clone-specific: Immunoprecipitation). 참조 보기
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Nakanishi K, Hirose S, Yoshimoto T, et al. Role and regulation of interleukin (IL)-2 receptor alpha and beta chains in IL-2-driven B-cell growth. Proc Natl Acad Sci U S A. 1992; 89(8):3551-3555. (Clone-specific: Blocking). 참조 보기
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Ohno H, Ono S, Hirayama N, Shimada S, Saito T. Preferential usage of the Fc receptor gamma chain in the T cell antigen receptor complex by gamma/delta T cells localized in epithelia. J Exp Med. 1994; 179(1):365-369. (Clone-specific: Flow cytometry). 참조 보기
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Rosmaraki EE, Douagi I, Roth C, Colucci F, Cumano A, Di Santo JP. Identification of committed NK cell progenitors in adult murine bone marrow. Eur J Immunol. 2001; 31(6):1900-1909. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). 참조 보기
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Suzuki H, Kundig TM, Furlonger C et al. Deregulated T cell activation and autoimmunity in mice lacking interleukin-2 receptor beta. Science. 1995; 268(5216):1472-1476. (Clone-specific: Blocking). 참조 보기
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Takeuchi Y, Tanaka T, Hamamura K et al. Expression and role of interleukin-2 receptor beta chain on CD4-CD8- T cell receptor alpha beta+ cells. Eur J Immunol. 1992; 22(11):2929-2935. (Clone-specific: Blocking). 참조 보기
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Tanaka T, Takeuchi Y, Shiohara T et al. In utero treatment with monoclonal antibody to IL-2 receptor beta-chain completely abrogates development of Thy-1+ dendritic epidermal cells. Int Immunol. 1992; 4(4):487-491. (Biology). 참조 보기
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Tanaka T, Tsudo M, Karasuyama H, et al. A novel monoclonal antibody against murine IL-2 receptor beta-chain. Characterization of receptor expression in normal lymphoid cells and EL-4 cells. J Immunol. 1991; 147(7):2222-2228. (Immunogen: Flow cytometry, Immunoprecipitation, Inhibition). 참조 보기
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Taniguchi T, Minami Y. The IL-2/IL-2 receptor system: a current overview. Cell. 1993; 73(1):5-8. (Biology). 참조 보기
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Zhang X, Sun S, Hwang I, Tough DF, Sprent J. Potent and selective stimulation of memory-phenotype CD8+ T cells in vivo by IL-15. Immunity. 1998; 8(5):591-599. (Clone-specific). 참조 보기
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