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- BD® AbSeq Assay
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- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Two-color flow cytometric analysis of TIGIT expression on mouse splenocytes. Mouse splenic leucocytes were stimulated (3 days) with concanavalin A (Con A). The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Hamster Anti-Mouse CD3e antibody (Cat. No. 553064/553063/561824) and either BD Horizon™ APC-R700 Mouse IgG1, κ Isotype Control (Cat. No. 564974; Left Panel) or BD Horizon APC-R700 Mouse Anti-Mouse TIGIT antibody (Cat. No. 565474; Right Panel). Two-color flow cytometric contour plots showing the correlated expression of TIGIT (or Ig Isotype control staining) versus CD3e were derived from gated events with the forward and side-light scattering characteristics of viable activated leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
BD Horizon™ APC-R700 Mouse Anti-Mouse TIGIT
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제품 고시
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
관련 제품
The 1G9 monoclonal antibody specifically binds to T cell immunoreceptor with Ig and ITIM domains (TIGIT) which is also known as, V-set and transmembrane domain-containing protein 3 (Vstm3). TIGIT is a 26 kDa, type I transmembrane glycoprotein that belongs to the poliovirus receptor (PVR) family. TIGIT is expressed on activated and memory CD4+ T cells and CD8+ T cells, follicular T helper cells, regulatory T cells, and NK cells. Its ligands include the poliovirus receptor (PVR, aka, CD155), poliovirus receptor-related protein 2 (PVRL2, aka, CD112), and poliovirus receptor-related protein 3 (PVRL3, CD113). TIGIT functions as a negative regulator of T cell and NK cell responses including proliferation, cytotoxicity, and the production of proinflammatory cytokines. TIGIT-deficient mice are reportedly more susceptible to developing autoimmune diseases in certain model systems.
This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor® 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor® 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor® (eg, 730/45-nm filter).
개발 참고 자료 (5)
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Foks AC, Ran IA, Frodermann V, Bot I et al. Agonistic anti-TIGIT treatment inhibits T cell responses in LDLr deficient mice without affecting atherosclerotic lesion development. PLoS ONE. 2013; 8(12):e83134. (Clone-specific: Flow cytometry). 참조 보기
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Joller N, Hafler JP, Brynedal B, et al. Cutting edge: TIGIT has T cell-intrinsic inhibitory functions. J Immunol. 2011; 186(3):1338-1342. (Immunogen: ELISA, Flow cytometry). 참조 보기
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Joller N, Lozano E, Burkett PR, et al.. Treg cells expressing the coinhibitory molecule TIGIT selectively inhibit proinflammatory Th1 and Th17 cell responses. Immunity. 2014; 40(4):569-581. (Biology). 참조 보기
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Levin SD, Taft DW, Brandt CS, et al. Vstm3 is a member of the CD28 family and an important modulator of T-cell function. Eur J Immunol. 2011; 41(4):902-915. (Biology). 참조 보기
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Yu X, Harden K, Gonzalez LC, Francesco M, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. Nat Immunol. 2009; 10(1):48-57. (Biology). 참조 보기
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